• Immunohistochemistry analysis of paraffin-embedded mouse lung using Twist Rabbit polyclonal antibody (STJ116194) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Twist Rabbit polyclonal antibody (STJ116194) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Twist Rabbit polyclonal antibody (STJ116194) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using Twist antibody (STJ116194) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TWIST1 antibody (1-100) (STJ116194)

SKU:
STJ116194

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Twist (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TWIST1
Gene ID: 7291
Uniprot ID: TWST1_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of Twist (NP_000465.1).
Immunogen Sequence: MMQDVSSSPVSPADDSLSNS EEEPDRQQPPSGKRGGRKRR SSRRSAGGGAGPGGAAGGGV GGGDEPGSPAQGKRGKKSAG CGGGGGAGGGGGSSSGGGSP
Tissue Specificity Subset of mesodermal cells.
Function Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-BMAL1 heterodimer.
Protein Name Twist-Related Protein 1
Class A Basic Helix-Loop-Helix Protein 38
Bhlha38
H-Twist
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-8878166
Reactome: R-HSA-8939902
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Twist-Related Protein 1 antibody
Anti-Class A Basic Helix-Loop-Helix Protein 38 antibody
Anti-Bhlha38 antibody
Anti-H-Twist antibody
Anti-TWIST1 antibody
Anti-BHLHA38 antibody
Anti-TWIST antibody

Information sourced from Uniprot.org

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