Developmental Biology | Research Area | St John's Laboratory

Western blot phosphorylated MEK-1 Thr292 in wild type and T292A mutant samples STJA0003684

Anti-Phospho-MAP2K1-Thr292 antibody (STJA0003684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Canine/Non-Human Primates/Rat

£488.50

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Western blot analysis of 3T3-EGF cells using Phospho-MEK-1 (T386) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K1-Thr386 antibody (344-393 aa) (STJ90585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot MEK 1 phospho-Thr386 immunolabeling in T47D cells with phosphatase control STJA0003685

Anti-Phospho-MAP2K1-Thr386 antibody (STJA0003685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Chicken/Non-Human Primates/Mouse/Xenopus

£488.50

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Western Blot MEK 1-2 phosphorylation at Ser218-Ser222 in NIH 3T3 cell lysates STJA0003683

Anti-Phospho-MAP2K1/2-Ser218/222 antibody (STJA0003683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Human/Bovine/Canine/Chicken/Non-Human Primates/Rat/Xenopus/Zebrafish

£488.50

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Western blot analysis of lysates from HepG2 cells using Phospho-MEK2-T394 Rabbit pAb (STJ22237). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K2-T394 antibody (STJ22237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K2-Thr394 antibody (261-310 aa) (STJ90329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HL60 cells, using Phospho-MEK6-S207 Rabbit pAb (STJ22247). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K6-S207 antibody (STJ22247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MAP2K6-Ser207 antibody (STJ90333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-MAPK1 antibody [M206] (STJA0005411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western Blot analysis of 1, HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MAPK12-Tyr185/182 antibody (STJA0009932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-MAPK14 antibody (250-300) (STJ502555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£497.50

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Anti-Phospho-MAPK14 antibody (250-300) {Biotin} (STJ502556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£551.50

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Anti-Phospho-MAPK14 antibody (250-300) {FITC} (STJ502557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£551.50

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-p38 MAPK-Y182 Rabbit polyclonal antibody (STJ11105887) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180 antibody (STJ11105887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180 antibody (147-196 aa) (STJ90362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180/Y182 antibody (151-200 aa) (STJ90498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/FC/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Guinea Pig

£49.00 - £261.00

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Western blot analysis of lysates from 1) PC-3, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-MAPK14-Tyr182 antibody (147-196 aa) (STJ90363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of human-stomach tissue. 1, p38 (phospho Tyr323) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-MAPK14-Tyr323 antibody (288-337 aa) (STJ90878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-Y182 Rabbit pAb (STJ22257) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-MAPK14-Y182 antibody (STJ22257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 + ERK2-Y187 Rabbit pAb (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-Y204 antibody (STJ22262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-MAPK8-Thr-183/Tyr-185 antibody [M268] (STJA0005456)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MCM2-S139 Rabbit pAb (STJ11103732) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. HeLa cells were treated by Serum-starvation overnight at 37 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MCM2-S139 antibody (STJ11103732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Phospho-MCM2 Ser108 antibody western blot rat spleen STJA0034547

Anti-Phospho-MCM2-Ser108 antibody [R01-7G9] (STJA0034547)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MCM2-Ser139 antibody (STJ196353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-MCM2 Ser27 antibody immunocytochemistry HeLa STJA0035076

Anti-Phospho-MCM2-Ser27 antibody [R08-4D8] (STJA0035076)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Phospho-MCM2 antibody western blot HeLa cells STJA0035685

Anti-Phospho-MCM2-Ser27 antibody [R88-9Q-1] (STJA0035685)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-MCM2 Ser40 antibody western blot HeLa A549 MCF-7 293T C2C12 STJA0035077

Anti-Phospho-MCM2-Ser40 antibody [R03-1B3] (STJA0035077)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-MCM2 Ser41 antibody western blot Jurkat C6 CHO-K1 HeLa STJA0033918

Anti-Phospho-MCM2-Ser41 antibody [R07-4C3] (STJA0033918)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat/Hamster

£133.50 - £281.50

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Anti-Phospho-MED1-Thr1032 antibody (STJA0010021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MED1-Thr1457 antibody (1423-1472 aa) (STJ91050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-MEF-2 (S408) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MEF2A-Ser408 antibody (374-423 aa) (STJ91020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEF2A (Phospho-Thr312) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2A-Thr312 antibody (279-328 aa) (STJ90323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-MEF-2 (T319) Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MEF2A-Thr319 antibody (296-345 aa) (STJ90324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from K-562 and MCF-7 cells, using Phospho-MEF2C-S396 Rabbit pAb (STJ22289) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MEF2C-S396 antibody (STJ22289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot MEF2C phospho-Ser222 detection in OCIAML2 lysate with phosphatase control STJA0003682

Anti-Phospho-MEF2C-Ser222 antibody (STJA0003682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Chicken/Guinea Pig/Non-Human Primates/Mouse/Rat/Sheep

£488.50

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Immunohistochemistry analysis of paraffin-embedded human heart, using MEF2C (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2C-Ser387 antibody (353-402 aa) (STJ91116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using MEF2C (Phospho-Ser396) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2C-Ser396 antibody (362-411 aa) (STJ90982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of K562 AD293 using Phospho-MEF-2D (S444) antibody. Antibody was diluted at 1:500

Anti-Phospho-MEF2D-Ser444 antibody (410-459 aa) (STJ90325)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-MEK1 Ser298 antibody immunocytochemistry HT-1080 STJA0034555

Anti-Phospho-MEK1-Ser298 antibody [R07-1I6] (STJA0034555)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit pAb (STJ11103753) at 1:1000 dilution. HeLa cells were treated by Nocodazole (100 ng/ml) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MEK1-T292 antibody (STJ11103753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Phospho-MEK1 antibody western blot C6 3T3 cells STJA0034556

Anti-Phospho-MEK1-Thr292 antibody [R01-5K8] (STJA0034556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

£133.50 - £281.50

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Phospho-MEK1 antibody western blot HeLa cells Nocodazole STJA0035668

Anti-Phospho-MEK1-Thr292 antibody [R52-4S-1] (STJA0035668)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MET-Ser985 antibody (STJA0010082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-Met (Phospho-Tyr1003) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MET-Tyr1003 antibody (976-1025 aa) (STJ91076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Met (Y1234) Polyclonal Antibody diluted at 1:1000 STJ90334

Anti-Phospho-MET-Tyr1234 antibody (1201-1250 aa) (STJ90334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MET-Tyr1234/1235 antibody (STJ196357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Met-Tyr1234/1235 antibody [PT0745R] (STJA0039221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-Phospho-MET-Tyr1235 antibody (STJA0009841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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