• Western blot analysis of extracts of K-562 and MCF-7 cells, using Phospho-MEF2C-S396 antibody (STJ22289) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of lysates from K-562 and MCF-7 cells, using Phospho-MEF2C-S396 Rabbit polyclonal antibody (STJ22289) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-MEF2C-S396 antibody (STJ22289)

SKU:
STJ22289

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MEF2C-S396 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MEF2C
Gene ID: 4208
Uniprot ID: MEF2C_HUMAN
Immunogen: A synthetic phosphorylated peptide around S396 of human MEF2C (NP_002388.2).
Immunogen Sequence: PVSPP
Tissue Specificity Expressed in brain and skeletal muscle.
Post Translational Modifications Phosphorylation on Ser-59 enhances DNA binding activity. Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation. Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity. Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
Function Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture. Isoforms that lack the repressor domain are more active than isoform 1.
Protein Name Myocyte-Specific Enhancer Factor 2c
Myocyte Enhancer Factor 2c
Database Links Reactome: R-HSA-198753
Reactome: R-HSA-2151201
Reactome: R-HSA-400253
Reactome: R-HSA-525793
Reactome: R-HSA-9022707
Reactome: R-HSA-9707616
Reactome: R-HSA-9733709
Cellular Localisation Nucleus
Cytoplasm
Sarcoplasm
Alternative Antibody Names Anti-Myocyte-Specific Enhancer Factor 2c antibody
Anti-Myocyte Enhancer Factor 2c antibody
Anti-MEF2C antibody

Information sourced from Uniprot.org

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