• Western blot of OCIAML2 lysate showing specific immunolabeling of the ~51 kDa MEF2C phosphorylated at Ser222 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-MEF2C-Ser222 antibody (STJA0003682)

SKU:
STJA0003682-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Bovine/Chicken/Guinea Pig/Non-Human Primates/Mouse/Rat/Sheep
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MEF2C-Ser222 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MEF2C
Gene ID: 4208
Uniprot ID: MEF2C_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser222 conjugated to KLH.
Tissue Specificity Expressed in brain and skeletal muscle.
Post Translational Modifications Phosphorylation on Ser-59 enhances DNA binding activity. Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation. Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity. Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
Function Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture. Isoforms that lack the repressor domain are more active than isoform 1.
Protein Name Myocyte-Specific Enhancer Factor 2c
Myocyte Enhancer Factor 2c
Database Links Reactome: R-HSA-198753
Reactome: R-HSA-2151201
Reactome: R-HSA-400253
Reactome: R-HSA-525793
Reactome: R-HSA-9022707
Reactome: R-HSA-9707616
Reactome: R-HSA-9733709
Cellular Localisation Nucleus
Cytoplasm
Sarcoplasm
Alternative Antibody Names Anti-Myocyte-Specific Enhancer Factor 2c antibody
Anti-Myocyte Enhancer Factor 2c antibody
Anti-MEF2C antibody

Information sourced from Uniprot.org

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