Anti-Phospho-MEF2C-Ser396 antibody (362-411 aa) (STJ90982)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MEF2C around the phosphorylation site of Ser396 at the amino acid range 362-411
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Myocyte-specific enhancer factor 2C-Ser396 (362-411 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MEF2C |
| Gene ID | 4208 |
| Uniprot ID | MEF2C_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MEF2C around the phosphorylation site of Ser396 at the amino acid range 362-411 |
| Immunogen Region | 362-411 aa |
| Specificity | Phospho-MEF-2C (S396) Polyclonal Antibody detects endogenous levels of MEF-2C protein only when phosphorylated at S396. |
Additional Info
| Post Translational Modifications | Phosphorylation on Ser-59 enhances DNA binding activity. Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation. Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity. Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. |
| Function | Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture. Isoforms that lack the repressor domain are more active than isoform 1. |
| Protein Name | Myocyte-Specific Enhancer Factor 2cMyocyte Enhancer Factor 2c |
| Database Links | Reactome: R-HSA-198753Reactome: R-HSA-2151201Reactome: R-HSA-400253Reactome: R-HSA-525793Reactome: R-HSA-9022707Reactome: R-HSA-9707616Reactome: R-HSA-9733709 |
| Cellular Localisation | NucleusCytoplasmSarcoplasm |
| Alternative Antibody Names | Anti-Myocyte-Specific Enhancer Factor 2c antibodyAnti-Myocyte Enhancer Factor 2c antibodyAnti-MEF2C antibody |
Information sourced from Uniprot.org