Anti-Phospho-MAPK8-Thr-183/Tyr-185 antibody [M268] (STJA0005456)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenClone M268 was generated from a dual phosphorylated peptide corresponding to amino acid residues surrounding Thr-183 and Tyr-185 in human JNK1. This sequence has high homology to the conserved site in rat and mouse JNK1 and JNK2.
General Information
| Short Description | Mouse monoclonal antibody anti-Phospho-JNK-Thr-183/Tyr-185 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Applications | WB/ICC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M268 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAPK8 |
| Gene ID | 5599 |
| Uniprot ID | MK08_HUMAN |
| Immunogen | Clone M268 was generated from a dual phosphorylated peptide corresponding to amino acid residues surrounding Thr-183 and Tyr-185 in human JNK1. This sequence has high homology to the conserved site in rat and mouse JNK1 and JNK2. |
| Specificity | This antibody detects 46 and 54 kDa* proteins corresponding to the apparent molecular mass of JNK1 and JNK2 on SDS-PAGE immunoblots of human A431 and HeLa cells, as well as rat PC12 cells treated with calyculin A. |
Additional Info
| Post Translational Modifications | Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1. Phosphorylated form is more concentrated at synapses than none-phosphorylated. |
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses. Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly. Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity. Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome. Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation. JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. |
| Protein Name | Mitogen-Activated Protein Kinase 8Map Kinase 8Mapk 8Jnk-46Stress-Activated Protein Kinase 1cSapk1cStress-Activated Protein Kinase Jnk1C-Jun N-Terminal Kinase 1 |
| Database Links | Reactome: R-HSA-111446Reactome: R-HSA-139910Reactome: R-HSA-193648Reactome: R-HSA-205043Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-450341Reactome: R-HSA-5693565Reactome: R-HSA-9007892Reactome: R-HSA-9673324Reactome: R-HSA-9725370 |
| Cellular Localisation | CytoplasmNucleusSynapseIn The Cortical NeuronsPredominantly Cytoplasmic And Associated With The Golgi Apparatus And Endosomal FractionIncreased Neuronal Activity Increases Phosphorylated Form At SynapsesColocalizes With Pou5f1 In The Nucleus |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 8 antibodyAnti-Map Kinase 8 antibodyAnti-Mapk 8 antibodyAnti-Jnk-46 antibodyAnti-Stress-Activated Protein Kinase 1c antibodyAnti-Sapk1c antibodyAnti-Stress-Activated Protein Kinase Jnk1 antibodyAnti-C-Jun N-Terminal Kinase 1 antibodyAnti-MAPK8 antibodyAnti-JNK1 antibodyAnti-PRKM8 antibodyAnti-SAPK1 antibodyAnti-SAPK1C antibody |
Information sourced from Uniprot.org