Anti-Phospho-MAPK12-Tyr185/182 antibody (STJA0009932)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human p38-Gamma/Delta (Phospho-Tyr185/182)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Mitogen-activated protein kinase 12-Tyr185/182 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAPK12 |
| Gene ID | 6300 |
| Uniprot ID | MK12_HUMAN |
| Immunogen | Synthesized peptide derived from human p38-Gamma/Delta (Phospho-Tyr185/182) |
| Specificity | This antibody detects endogenous levels of p38-Gamma/Delta (Phospho-Tyr185/182) at Human, Mouse, Rat |
Additional Info
| Tissue Specificity | Highly expressed in skeletal muscle and heart. |
| Post Translational Modifications | Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K3/MKK3 and MAP2K6/MKK6, which activates the enzyme. Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway. |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes.and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration. |
| Protein Name | Mitogen-Activated Protein Kinase 12Map Kinase 12Mapk 12Extracellular Signal-Regulated Kinase 6Erk-6Mitogen-Activated Protein Kinase P38 GammaMap Kinase P38 GammaStress-Activated Protein Kinase 3 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-171007Reactome: R-HSA-2151209Reactome: R-HSA-4420097Reactome: R-HSA-525793Reactome: R-HSA-5675221 |
| Cellular Localisation | CytoplasmNucleusMitochondrionMitochondrial When Associated With Sh3bp5In Skeletal Muscle Colocalizes With Snta1 At The Neuromuscular Junction And Throughout The Sarcolemma |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 12 antibodyAnti-Map Kinase 12 antibodyAnti-Mapk 12 antibodyAnti-Extracellular Signal-Regulated Kinase 6 antibodyAnti-Erk-6 antibodyAnti-Mitogen-Activated Protein Kinase P38 Gamma antibodyAnti-Map Kinase P38 Gamma antibodyAnti-Stress-Activated Protein Kinase 3 antibodyAnti-MAPK12 antibodyAnti-ERK6 antibodyAnti-SAPK3 antibody |
Information sourced from Uniprot.org