Cell Cycle | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using RFC2 antibody (STJ28929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RFC2 antibody (38-297) (STJ28929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

Choose Options

RFC2 antibody western blot K562 HeLa STJA0037527

Anti-RFC2 antibody (STJA0037527)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Western blot analysis of extracts of various cell lines, using RFC3 antibody (STJ25342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RFC3 antibody (1-280) (STJ25342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of various cells using RFC3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-RFC3 antibody (178-227 aa) (STJ95426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Anti-RFC4 antibody (204-363) (STJ27438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

Choose Options

Anti-RFC4 antibody (STJA0007432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

Choose Options

Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RFC5 antibody (110-190 aa) (STJ190417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

Choose Options

Western blot analysis of various lysates using RFC5 Rabbit pAb (STJ110359) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-RFC5 antibody (181-340) (STJ110359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of extracts of various cell lines, using RFWD2 antibody (STJ112492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RFWD2 antibody (582-731) (STJ112492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

Choose Options

Western blot analysis of extracts of various cell lines, using RMI1 Antibody (STJ25361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RMI1 antibody (1-350) (STJ25361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

Choose Options

Anti-RMI1 antibody (STJA0007006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

Choose Options

Western blot analysis of various lysates using <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RMI2 antibody (1-147) (STJ110821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

Choose Options

Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RMI2 antibody (73-123 aa) (STJ193992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

Choose Options

POLR2C antibody western blot Jurkat lysates STJA0037159

Anti-RNA Polymerase II Subunit B3 antibody [R09-8K6] (STJA0037159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

POLR2E antibody western blot K562 C6 3T3 HeLa STJA0034741

Anti-RNA Polymerases I/II/III Subunit ABC1 antibody [R05-8J5] (STJA0034741)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Western blot analysis of extracts of various cell lines, using RNF168 antibody (STJ25369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RNF168 antibody (300-571) (STJ25369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

Choose Options

Anti-RNF168 antibody (STJA0007063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various lysates using RNF219 Rabbit pAb (STJ11107510) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RNF219 antibody (600 aa) (STJ11107510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of various lysates using RNF8 Rabbit mAb (STJ11107286) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-RNF8 antibody (1-260 aa) [SRM5375] (STJ11107286)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

Choose Options

Western blot analysis of various lysates, using RNF8 antibody (STJ11105728) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RNF8 antibody (230-485) (STJ11105728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Anti-RNF8 antibody (STJA0006845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

RNF8 antibody western blot human fetal kidney STJA0036584

Anti-RNF8 antibody [R06-6O7] (STJA0036584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$184.23 - $388.47

Choose Options

Anti-RNF8 antibody [SRM13243] (STJ11107973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

Choose Options

Western blot analysis of extracts of various cell lines, using RPA70/RPA1 rabbit monoclonal antibody (STJ11101855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPA1 antibody (1-100) [S5MR] (STJ11101855)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Rat

$163.53 - $719.67

Choose Options

Immunohistochemistry analysis of RPA70/RPA1 in paraffin-embedded human uterus using RPA70/RPA1 Rabbit polyclonal antibody (STJ114904) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-RPA1 antibody (1-240) (STJ114904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$152.49 - $697.59

Choose Options

RPA70 antibody western blot HeLa A431 MCF-7 COS7 HT-1080 K562 STJA0012829

Anti-RPA1 antibody (317-616 aa) [8C3-D12-H10] (STJA0012829)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Monkey/Mouse/Rat

$184.23 - $388.47

Choose Options

Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-RPA1 antibody (STJA0010246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Monkey/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot detection of RPA70 in Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-RPA1 antibody [8C3-D12-H10] (STJ99205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Monkey/Mouse/Rat

$205.62 - $496.80

Choose Options

Immunofluorescence analysis of U-2 OS cells using RPA2 Rabbit polyclonal antibody (STJ11105520) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RPA2 antibody (1-270) (STJ11105520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

Choose Options

Immunofluorescence analysis of HeLa cells, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-RPA2 antibody (10-59 aa) (STJ95535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

Choose Options

Western blot detection of RPA32/RPA2 in MCF7, Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s.

Anti-RPA2 antibody [3E7-B5-F2] (STJ99210)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human

$205.62 - $496.80

Choose Options

Anti-RPA2 antibody [SRP14464] (STJ11109194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of lysate; antibody was diluted at 1:2000. Secondary antibody was diluted at STJ98675

Anti-RPA3 antibody (30-80 aa) (STJ98675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

Choose Options

RPA32/RPA2 antibody western blot MCF-7 HeLa HUVEC STJA0032447

Anti-RPA32 antibody [3E7-B5-F2] (STJA0032447)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

Choose Options

RPA32 antibody western blot 3T3 lysates STJA0034821

Anti-RPA32 antibody [R07-4D1] (STJA0034821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat/Hamster

$184.23 - $388.47

Choose Options

Anti-RPA32/RPA2 antibody [SRM14911] (STJ11109641)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

Choose Options

Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RPA4 antibody (STJ193094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

Choose Options

RPA70 antibody western blot C6 COS7 K562 HeLa STJA0038223

Anti-RPA70 antibody [8C3-D12-H10] (STJA0038223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Monkey/Mouse/Rat

$184.23 - $388.47

Choose Options

RPA70 antibody western blot A549 lysates STJA0034822

Anti-RPA70 antibody [R04-7B7] (STJA0034822)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

Choose Options

RPA70 antibody western blot HEK293 lysates STJA0036621

Anti-RPA70 antibody [R05-7P8] (STJA0036621)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

$184.23 - $388.47

Choose Options

Anti-Rpb1 antibody [PT0570R] (STJA0039415)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA/CHIP/Cut&Tag

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

Choose Options

Western blot analysis of various lysates using RPB3/POLR2C Rabbit mAb (STJ11103678) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-RPB3/POLR2C antibody (1-100) [S8MR] (STJ11103678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

Choose Options

Western blot analysis of extracts of various cell lines, using RPS27 antibody (STJ28812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS27 antibody (1-84) (STJ28812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

Choose Options

Immunohistochemistry analysis of paraffin-embedded human lung cancer (left) and human brain (right) tissues with DAB staining using Ribosomal Protein S27 monoclonal antibody.

Anti-RPS27 antibody [4A12] (STJ98360)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

$205.62 - $496.80

Choose Options

Confocal immunofluorescence analysis of Hela cells (A) , BCBL-1 cells (B) and L1210 cells (C) using Ribosomal Protein S27 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Anti-RPS27 antibody [7E3] (STJ98361)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/ELISA

Reactivity :

Human

$205.62 - $496.80

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse kidney using RPS27A antibody (STJ116826) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-RPS27A antibody (1-156) (STJ116826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of extracts of various cell lines, using RPS27A antibody (STJ25406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS27A antibody (1-156) (STJ25406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Anti-RPS27A antibody (STJA0007619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options