| Host: | Rabbit |
| Applications: | WB/IF/IP |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-RFC4 (204-363) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IF/ICC 1:50-1:100IP 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RFC4 |
| Gene ID: | 5984 |
| Uniprot ID: | RFC4_HUMAN |
| Immunogen Region: | 204-363 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1). |
| Immunogen Sequence: | DKIQQQRLLDIAKKENVKIS DEGIAYLVKVSEGDLRKAIT FLQSATRLTGGKEITEKVIT DIAGVIPAEKIDGVFAACQS GSFDKLEAVVKDLIDEGHAA TQLVNQLHDVVVENNLSDKQ KSIITEKLAEVDKCLADGAD EHLQLISLCATVMQQLSQNC |
| Function | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. |
| Protein Name | Replication Factor C Subunit 4Activator 1 37 Kda SubunitA1 37 Kda SubunitActivator 1 Subunit 4Replication Factor C 37 Kda SubunitRf-C 37 Kda SubunitRfc37 |
| Database Links | Reactome: R-HSA-110312Reactome: R-HSA-110314Reactome: R-HSA-110320Reactome: R-HSA-174411Reactome: R-HSA-176187Reactome: R-HSA-5651801Reactome: R-HSA-5655862Reactome: R-HSA-5656121Reactome: R-HSA-5656169Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6804756Reactome: R-HSA-69091Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Replication Factor C Subunit 4 antibodyAnti-Activator 1 37 Kda Subunit antibodyAnti-A1 37 Kda Subunit antibodyAnti-Activator 1 Subunit 4 antibodyAnti-Replication Factor C 37 Kda Subunit antibodyAnti-Rf-C 37 Kda Subunit antibodyAnti-Rfc37 antibodyAnti-RFC4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA) ) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit polyclonal antibody (STJ11100002) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89075/357532/STJ11100002_1__24167.1713121647.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA) ) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit polyclonal antibody (STJ11100002) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
