• Western blot analysis of extracts of various cell lines, using RPA70/RPA1 rabbit monoclonal antibody (STJ11101855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g RPA70/RPA1 antibody (STJ11101855). Western blot was performed from the immunoprecipitate using RPA70/RPA1 antibody (STJ11101855) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RPA70/RPA1 rabbit monoclonal antibody antibody (STJ11101855) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RPA1 antibody (1-100) [S5MR] (STJ11101855)

SKU:
STJ11101855

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RPA70 (1-100) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:500-1:1000
ChIP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPA1
Gene ID: 6117
Uniprot ID: RFA1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694).
Immunogen Sequence: MVGQLSEGAIAAIMQKGDTN IKPILQVINIRPITTGNSPP RYRLLMSDGLNTLSSFMLAT QLNPLVEEEQLSSNCVCQIH RFIVNTLKDGRRVVILMELE
Post Translational Modifications DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination. Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated by SENP6.
Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. Plays a role in telomere maintenance. As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Protein Name Replication Protein A 70 Kda Dna-Binding Subunit
Rp-A P70
Replication Factor A Protein 1
Rf-A Protein 1
Single-Stranded Dna-Binding Protein Cleaved Into - Replication Protein A 70 Kda Dna-Binding Subunit - N-Terminally Processed
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174437
Reactome: R-HSA-176187
Reactome: R-HSA-3108214
Reactome: R-HSA-3371453
Reactome: R-HSA-3371511
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696395
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6783310
Reactome: R-HSA-6804756
Reactome: R-HSA-68962
Reactome: R-HSA-69166
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Pml Body
Enriched In Pml Bodies In Cells Displaying Alternative Lengthening Of Their Telomeres
Alternative Antibody Names Anti-Replication Protein A 70 Kda Dna-Binding Subunit antibody
Anti-Rp-A P70 antibody
Anti-Replication Factor A Protein 1 antibody
Anti-Rf-A Protein 1 antibody
Anti-Single-Stranded Dna-Binding Protein Cleaved Into - Replication Protein A 70 Kda Dna-Binding Subunit - N-Terminally Processed antibody
Anti-RPA1 antibody
Anti-REPA1 antibody
Anti-RPA70 antibody

Information sourced from Uniprot.org

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