• Western blot analysis of extracts of various cell lines, using RPS27A antibody (STJ25406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using RPS27A antibody (STJ25406) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using RPS27A antibody (STJ25406) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RPS27A antibody (STJ25406) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of MCF7 cells using RPS27A rabbit polyclonal antibody (STJ25406) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RPS27A antibody (1-156) (STJ25406)

SKU:
STJ25406

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS27A (1-156) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS27A
Gene ID: 6233
Uniprot ID: RS27A_HUMAN
Immunogen Region: 1-156
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPS27A (NP_001170884.1).
Immunogen Sequence: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGAKKR KKKSYTTPKKNKHKRKKVKL AVLKYYKVDENGKISRLRRE CPSDECGAGVFMASHFDRHY CGKCCLTYCFNKPEDK
Post Translational Modifications Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30. Ubiquitin: Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones. Ubiquitin: (Microbial infection) Mono-ADP-ribosylated at Thr-66 by the C.violaceum CteC virulence factor. ADP-ribosylation causes the shutdown of polyubiquitin synthesis and disrupts the recognition and reversal of polyubiquitin. Small ribosomal subunit protein eS31: Monoubiquitinated at Lys-107 and Lys-113 by RNF25 in response to ribosome collisions (ribosome stalling): ubiquitination promotes subsequent activation of RNF14, leading to EEF1A1 ubiquitination and degradation and rescue of stalled ribosomes. Deubiquitination at Lys-113 by USP16 is required for maturation of the 40S ribosomal complex.
Function Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair.Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation.Lys-29-linked is involved in proteotoxic stress response and cell cycle.Lys-33-linked is involved in kinase modification.Lys-48-linked is involved in protein degradation via the proteasome.Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Small ribosomal subunit protein eS31: Component of the 40S subunit of the ribosome. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Ubiquitin-Ribosomal Protein Es31 Fusion Protein
Ubiquitin Carboxyl Extension Protein 80 Cleaved Into - Ubiquitin - Small Ribosomal Subunit Protein Es31
40s Ribosomal Protein S27a
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-1169091
Reactome: R-HSA-1169408
Reactome: R-HSA-1234176
Reactome: R-HSA-1236382
Reactome: R-HSA-1236974
Reactome: R-HSA-1253288
Reactome: R-HSA-1295596
Reactome: R-HSA-1358803
Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-162588
Reactome: R-HSA-168638
Reactome: R-HSA-168927
Reactome: R-HSA-168928
Reactome: R-HSA-174048
Reactome: R-HSA-174084
Reactome: R-HSA-174113
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-174490
Reactome: R-HSA-175474
Reactome: R-HSA-179409
Reactome: R-HSA-1799339
Reactome: R-HSA-180534
Reactome: R-HSA-180585
Reactome: R-HSA-182971
Reactome: R-HSA-187577
Reactome: R-HSA-192823
Reactome: R-HSA-195253
Reactome: R-HSA-201681
Reactome: R-HSA-202424
Reactome: R-HSA-205043
Reactome: R-HSA-209543
Reactome: R-HSA-209560
Reactome: R-HSA-211733
Reactome: R-HSA-2122947
Reactome: R-HSA-2122948
Reactome: R-HSA-2173788
Reactome: R-HSA-2173791
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-2408557
Reactome: R-HSA-2467813
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-2565942
Reactome: R-HSA-2644606
Reactome: R-HSA-2672351
Reactome: R-HSA-2691232
Reactome: R-HSA-2871837
Reactome: R-HSA-2894862
Reactome: R-HSA-2979096
Reactome: R-HSA-3134975
Reactome: R-HSA-3322077
Reactome: R-HSA-349425
Reactome: R-HSA-3769402
Reactome: R-HSA-3785653
Reactome: R-HSA-382556
Reactome: R-HSA-400253
Reactome: R-HSA-445989
Reactome: R-HSA-450302
Reactome: R-HSA-450321
Reactome: R-HSA-450408
Reactome: R-HSA-4608870
Reactome: R-HSA-4641257
Reactome: R-HSA-4641258
Reactome: R-HSA-4641263
Reactome: R-HSA-5205685
Reactome: R-HSA-532668
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5632684
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5658442
Reactome: R-HSA-5668541
Reactome: R-HSA-5675221
Reactome: R-HSA-5675482
Reactome: R-HSA-5676590
Reactome: R-HSA-5678895
Reactome: R-HSA-5684264
Reactome: R-HSA-5685942
Reactome: R-HSA-5687128
Reactome: R-HSA-5689603
Reactome: R-HSA-5689877
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-5689901
Reactome: R-HSA-5693565
Reactome: R-HSA-5693607
Reactome: R-HSA-5696394
Reactome: R-HSA-5696395
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6783310
Reactome: R-HSA-6791226
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-6804760
Reactome: R-HSA-6807004
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69231
Reactome: R-HSA-69481
Reactome: R-HSA-69541
Reactome: R-HSA-69601
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-75815
Reactome: R-HSA-8849469
Reactome: R-HSA-8852276
Reactome: R-HSA-8854050
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8863795
Reactome: R-HSA-8866427
Reactome: R-HSA-8866652
Reactome: R-HSA-8866654
Reactome: R-HSA-8875360
Reactome: R-HSA-8876493
Reactome: R-HSA-8939236
Reactome: R-HSA-8939902
Reactome: R-HSA-8941858
Reactome: R-HSA-8948747
Reactome: R-HSA-8948751
Reactome: R-HSA-8951664
Reactome: R-HSA-901032
Reactome: R-HSA-9010553
Reactome: R-HSA-9013507
Reactome: R-HSA-9013973
Reactome: R-HSA-9014325
Reactome: R-HSA-9020702
Reactome: R-HSA-9033241
Reactome: R-HSA-909733
Reactome: R-HSA-912631
Reactome: R-HSA-917729
Reactome: R-HSA-917937
Reactome: R-HSA-936440
Reactome: R-HSA-936964
Reactome: R-HSA-937039
Reactome: R-HSA-937041
Reactome: R-HSA-937042
Reactome: R-HSA-937072
Reactome: R-HSA-9604323
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9633012
Reactome: R-HSA-9636383
Reactome: R-HSA-9637628
Reactome: R-HSA-9645460
Reactome: R-HSA-9646399
Reactome: R-HSA-9648002
Reactome: R-HSA-9664873
Reactome: R-HSA-9680350
Reactome: R-HSA-9683683
Reactome: R-HSA-9692916
Reactome: R-HSA-9694493
Reactome: R-HSA-9705462
Reactome: R-HSA-9705671
Reactome: R-HSA-9706369
Reactome: R-HSA-9706377
Reactome: R-HSA-9708530
Reactome: R-HSA-9735869
Reactome: R-HSA-975110
Reactome: R-HSA-975144
Reactome: R-HSA-975163
Reactome: R-HSA-9754678
Reactome: R-HSA-9755511
Reactome: R-HSA-9758274
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Reactome: R-HSA-9762114
Reactome: R-HSA-977225
Reactome: R-HSA-983168
Cellular Localisation Small Ribosomal Subunit Protein Es31: Nucleus
Nucleolus
Ubiquitin: Cytoplasm
Nucleus
Alternative Antibody Names Anti-Ubiquitin-Ribosomal Protein Es31 Fusion Protein antibody
Anti-Ubiquitin Carboxyl Extension Protein 80 Cleaved Into - Ubiquitin - Small Ribosomal Subunit Protein Es31 antibody
Anti-40s Ribosomal Protein S27a antibody
Anti-RPS27A antibody
Anti-UBA80 antibody
Anti-UBCEP1 antibody

Information sourced from Uniprot.org

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