| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RFWD2 (582-731) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
COP1 |
| Gene ID: |
64326 |
| Uniprot ID: |
COP1_HUMAN |
| Immunogen Region: |
582-731 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human RFWD2 (NP_071902.2). |
| Immunogen Sequence: |
YYDLRNTKQPIMVFKGHRKA VSYAKFVSGEEIVSASTDSQ LKLWNVGKPYCLRSFKGHIN EKNFVGLASNGDYIACGSEN NSLYLYYKGLSKTLLTFKFD TVKSVLDKDRKEDDTNEFVS AVCWRALPDGESNVLIAANS QGTIKVLELV |
| Tissue Specificity | Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. |
| Post Translational Modifications | Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable). |
| Protein Name | E3 Ubiquitin-Protein Ligase Cop1Constitutive Photomorphogenesis Protein 1 HomologHcop1Ring Finger And Wd Repeat Domain Protein 2Ring Finger Protein 200Ring-Type E3 Ubiquitin Transferase Rfwd2 |
| Database Links | Reactome: R-HSA-349425Reactome: R-HSA-8951664 |
| Cellular Localisation | Nucleus SpeckleCytoplasmIn The NucleusIt Forms Nuclear Speckles |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Cop1 antibodyAnti-Constitutive Photomorphogenesis Protein 1 Homolog antibodyAnti-Hcop1 antibodyAnti-Ring Finger And Wd Repeat Domain Protein 2 antibodyAnti-Ring Finger Protein 200 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rfwd2 antibodyAnti-COP1 antibodyAnti-RFWD2 antibodyAnti-RNF200 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/93884/375089/STJ114797_1__72218.1713141770.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
