• Western blot analysis of extracts of various cell lines, using RFC3 antibody (STJ25342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RFC3 antibody (1-280) (STJ25342)

SKU:
STJ25342

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RFC3 (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RFC3
Gene ID: 5983
Uniprot ID: RFC3_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RFC3 (NP_002906.1).
Immunogen Sequence: MSLWVDKYRPCSLGRLDYHK EQAAQLRNLVQCGDFPHLLV YGPSGAGKKTRIMCILRELY GVGVEKLRIEHQTITTPSKK KIEISTIASNYHLEVNPSDA GNSDRVVIQEMLKTVAQSQQ LETNSQRDFKVVLLTEVDKL TKDAQHALRRTMEKYMSTCR LILCCNSTSKVIPPIRSRCL AVRVPAPSIEDICHVLSTVC KKEGLNLPSQLAHRLAEKSC RNLRKALLMCEACRVQQYP
Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
Protein Name Replication Factor C Subunit 3
Activator 1 38 Kda Subunit
A1 38 Kda Subunit
Activator 1 Subunit 3
Replication Factor C 38 Kda Subunit
Rf-C 38 Kda Subunit
Rfc38
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174411
Reactome: R-HSA-176187
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804756
Reactome: R-HSA-69091
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Replication Factor C Subunit 3 antibody
Anti-Activator 1 38 Kda Subunit antibody
Anti-A1 38 Kda Subunit antibody
Anti-Activator 1 Subunit 3 antibody
Anti-Replication Factor C 38 Kda Subunit antibody
Anti-Rf-C 38 Kda Subunit antibody
Anti-Rfc38 antibody
Anti-RFC3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance