Autophagy | Research Area | St John's Laboratory

Western blot analysis of lysates from C6 cells using MAP1LC3B Rabbit polyclonal antibody (STJ11104549) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAP1LC3B antibody (1-50) (STJ11104549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

Choose Options

Anti-MAP1LC3B antibody (2-107) [S4MR] (STJ11101754)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

Choose Options

Anti-MAP1LC3B antibody (STJ13101341)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

$445.05

Choose Options

Jia, Wei‐Qiang, et al. "Verbascoside inhibits progression of glioblastoma cells by promoting Let‐7g‐5p and down‐regulating HMGA2 via Wnt/beta‐catenin signalling blockade. " Journal of cellular and molecular medicine 24. 5 (2020) : 2901-2916.

Anti-MAP1LC3B antibody (STJ197355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using LC3B Mouse mAb diluted at 1:200.

Anti-MAP1LC3B antibody [9H5] (STJ97748)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Cow

$67.62 - $360.18

Choose Options

Western blot analysis of various lysates using MAP1LC3C Rabbit pAb (STJ117859) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-MAP1LC3C antibody (1-147) (STJ117859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MAP1LC3C antibody (71-120 aa) (STJ193135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

Choose Options

Anti-MAP1LC3C antibody (STJ13101335)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

$445.05

Choose Options

Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MFN1 antibody (163-213 aa) (STJ193472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various lysates, using Mitofusin-1/MFN1 Rabbit pAb (STJ111922) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-MFN1 antibody (622-741) (STJ111922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Anti-MFN1 antibody [PT0839R] (STJA0039186)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

Choose Options

Western blot analysis of various cells using Mfn2 Polyclonal Antibody diluted at 1：1000

Anti-MFN2 antibody (354-403 aa) (STJ94105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Immunohistochemistry of rat brain with Anti-MFN2 primary antibody (STJ501759) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-MFN2 antibody (543-593) (STJ501759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

Choose Options

Anti-MFN2 antibody (543-593) {Biotin} (STJ501760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

Choose Options

Anti-MFN2 antibody (543-593) {FITC} (STJ501761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

Choose Options

Immunofluorescence analysis of HeLa cells using Mitofusin 2 Rabbit polyclonal antibody (STJ114644) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-MFN2 antibody (70-260) (STJ114644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Anti-Microtubule-associated protein 1 light chain 3 gamma antibody (N-Term) (STJA0040902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/IHC-F/WB

Reactivity :

Human

$729.33

Choose Options

Anti-Microtubule-associated proteins 1A/1B light chain 3A antibody (11-23 aa) [BS405] (STJA0040788)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ICC/WB

Reactivity :

Human/Mouse/Rat

$729.33

Choose Options

Anti-Microtubule-associated proteins 1A/1B light chain 3A antibody (N-Term) (STJA0040910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/WB

Reactivity :

Human/Rat

$729.33

Choose Options

Anti-Microtubule-associated proteins 1A/1B light chain 3B antibody (N-Term) (STJA0040931)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/WB

Reactivity :

Human/Rat

$729.33

Choose Options

ICC staining HeLa cells using antibody mitochondrial localization with Mortalin co-stain STJA0040640

Anti-Mitochondrial Import Receptor Subunit TOM20 Homolog antibody (Cyt Dom) (STJA0040640)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ICC/IHC/WB

Reactivity :

Human/Mouse/Rat

$406.41 - $970.83

Choose Options

Anti-Mitofusin 1 antibody [R02-2M-8] (STJA0033065)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$296.01 - $425.73

Choose Options

Mitofusin 1 antibody western blot K562 cells STJA0036252

Anti-Mitofusin 1 antibody [R08-3E5] (STJA0036252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/FC

Reactivity :

Human

$184.23 - $388.47

Choose Options

Mitofusin 2 antibody western blot HeLa mouse kidney STJA0035453

Anti-Mitofusin 2 antibody [R56-8V-9] (STJA0035453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Anti-Mitofusin 2 antibody [SRM13073] (STJ11107803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

Choose Options

Anti-Mitofusin-1/MFN1 antibody [SRM14462] (STJ11109192)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$163.53 - $719.67

Choose Options

Immunofluorescence analysis of U2OS cells using MLST8 antibody (STJ115560).

Anti-MLST8 antibody (1-326) (STJ115560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of extracts of various cell lines, using MLST8 antibody (STJ24568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MLST8 antibody (1-326) (STJ24568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

Choose Options

Anti-MLST8 antibody (100-180 aa) (STJ192498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

Choose Options

Western blot analysis of 1) Hela Cell lysate, 2) Rat Kidney Tissue lysate, 3) 3T3 Cell lysate using GβL Rabbit pAb diluted at 1:1000.

Anti-MLST8 antibody (44-94) (STJA0006727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Immunohistochemical analysis of paraffin-embedded Human Heart Tissue using ILK1 Rabbit polyclonal antibody diluted at 1:200.

Anti-MLST8 antibody (89-139) (STJA0006728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC

Reactivity :

Human

$67.62 - $360.18

Choose Options

ICC IF of MP1 LAMTOR3 in HaLa cells with Alexa Fluor 488 and DAPI staining

Anti-MP1/LAMTOR3 antibody (1-124aa) [AT1F10] (STJA0041876)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$302.91 - $458.85

Choose Options

Anti-MTERF3 antibody (STJA0009350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Western blot analysis of various lysates using MTERFD1 Rabbit pAb (STJ111846) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MTERFD1 antibody (69-300) (STJ111846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

Choose Options

Western blot analysis of various lysates using MTMR14 Rabbit pAb (STJ11106808) at 1:1000 dilution. <br> Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 90s.

Anti-MTMR14 antibody (1-280 aa) (STJ11106808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

Choose Options

Immunofluorescence analysis of NIH/3T3 cells using MTMR14 Rabbit polyclonal antibody (STJ29616) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MTMR14 antibody (335-500) (STJ29616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Anti-MTMR14 antibody (STJA0008335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

Choose Options

Western blot analysis of lysates from wild type (WT) and MTMR3 knockdown (KD) 293T (KD) cells, using MTMR3 Rabbit polyclonal antibody (STJ112702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MTMR3 antibody (640-810) (STJ112702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

Choose Options

Anti-MTMR3 antibody (STJA0008857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$67.62 - $360.18

Choose Options

Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug mTOR Rabbit polyclonal antibody (STJ29884 1:70). Western blot was performed from the immunoprecipitate using mTOR Rabbit polyclonal antibody (STJ29884) at a dilition of 1:500.

Anti-mTOR antibody (1-300) (STJ29884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options

Western blot analysis of extracts of various cell lines, using mTOR antibody (STJ113771) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-mTOR antibody (2400-2500) (STJ113771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

Choose Options

Western blot analysis of various cells using mTOR Polyclonal Antibody

Anti-MTOR antibody (2447-2496) (STJ94278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig

$67.62 - $360.18

Choose Options

Anti-mTOR antibody [PT0351R] (STJA0039470)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

Choose Options

mTOR antibody western blot HeLa C2C12 rat brain STJA0033064

Anti-mTOR antibody [R02-9G-6] (STJA0033064)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$296.01 - $425.73

Choose Options

mTOR antibody western blot SH-SY5Y rat brain STJA0035711

Anti-mTOR antibody [R07-8H4] (STJA0035711)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

Choose Options

Western blot analysis of lysates from rat pancreas, using MVB12A Rabbit polyclonal antibody (STJ118378) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-MVB12A antibody (1-273) (STJ118378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$152.49 - $697.59

Choose Options

Anti-MVB12A antibody (11-61) (STJA0006476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

Choose Options

Immunofluorescence analysis of PC-12 cells using NBR1 Rabbit polyclonal antibody (STJ24692) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NBR1 antibody (1-966) (STJ24692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

Choose Options