• Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MLST8 antibody (100-180 aa) (STJ192498)

SKU:
STJ192498

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Target of rapamycin complex subunit LST8 (100-180 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLST8
Gene ID: 64223
Uniprot ID: LST8_HUMAN
Immunogen Region: 100-180 aa
Specificity: LST8 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from the human protein at the amino acid range 100-180
Post Translational Modifications Phosphorylation at Thr-51 by CDK1 promotes ubiquitination by the SCF(FBXW7) complex, followed by degradation. Ubiquitination by the SCF(FBXW7) and SCF(FBXW11) complexes following phosphorylation at Thr-51 by CDK1, leads to its degradation by the proteasome.
Function Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy. The mTORC1 complex is inhibited in response to starvation and amino acid depletion. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Protein Name Target Of Rapamycin Complex Subunit Lst8
Torc Subunit Lst8
G Protein Beta Subunit-Like
Gable
Protein Gbetal
Mammalian Lethal With Sec13 Protein 8
Mlst8
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-1632852
Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-3371571
Reactome: R-HSA-380972
Reactome: R-HSA-389357
Reactome: R-HSA-5218920
Reactome: R-HSA-5628897
Reactome: R-HSA-5674400
Reactome: R-HSA-6804757
Reactome: R-HSA-8943724
Reactome: R-HSA-9639288
Cellular Localisation Lysosome Membrane
Cytoplasm
Targeting To Lysosomal Membrane Depends On Amino Acid Availability: Mtorc1 Is Recruited To Lysosome Membranes Via Interaction With Gtp-Bound Form Of Raga/Rraga (Or Ragb/Rragb) In Complex With The Gdp-Bound Form Of Ragc/Rragc (Or Ragd/Rragd)
Promoting Its Mtorc1 Recruitment To The Lysosomes
Alternative Antibody Names Anti-Target Of Rapamycin Complex Subunit Lst8 antibody
Anti-Torc Subunit Lst8 antibody
Anti-G Protein Beta Subunit-Like antibody
Anti-Gable antibody
Anti-Protein Gbetal antibody
Anti-Mammalian Lethal With Sec13 Protein 8 antibody
Anti-Mlst8 antibody
Anti-MLST8 antibody
Anti-GBL antibody
Anti-LST8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance