Anti-Mitofusin 1 antibody [R08-3E5] (STJA0036252)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Mitofusin1
General Information
| Short Description | Rabbit monoclonal anti-Mitofusin 1 for use in WB, IP and FC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/FC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-3E5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IP 1:20FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MFN1 |
| Gene ID | 55669 |
| Uniprot ID | MFN1_HUMAN |
| Immunogen | A synthesized peptide derived from human Mitofusin1 |
Additional Info
| Tissue Specificity | Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. |
| Post Translational Modifications | Ubiquitinated by non-degradative ubiquitin by PRKN. Deubiquitination by USP30 inhibits mitochondrial fusion. Ubiquitinated by MARCHF5. When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN. |
| Function | Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity. |
| Protein Name | Mitofusin-1Fzo HomologTransmembrane Gtpase Mfn1 |
| Database Links | Reactome: R-HSA-5205685Reactome: R-HSA-9013419Reactome: R-HSA-983231 |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane ProteinIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Mitofusin-1 antibodyAnti-Fzo Homolog antibodyAnti-Transmembrane Gtpase Mfn1 antibodyAnti-MFN1 antibody |
Information sourced from Uniprot.org