Anti-MAP1LC3C antibody (STJ13101335)
SPECIFICATIONS
ClonalityPolyclonal
HostSheep
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from human microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C, MAP1A/MAP1B LC3 C) conjugated to an immunogenic carrier protein has been used as the immunogen.
General Information
| Short Description | Sheep polyclonal anti-MAP1LC3C for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Sheep |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | MAP1LC3C |
| Gene ID | 440738 |
| Uniprot ID | MLP3C_HUMAN |
| Immunogen | A synthetic peptide from human microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C, MAP1A/MAP1B LC3 C) conjugated to an immunogenic carrier protein has been used as the immunogen. |
| Specificity | Specific for MAP1LC3C. |
Additional Info
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-96 and Ser-98 by TBK1 prevents interaction with ATG4 (ATG4A, ATG4B, ATG4C or ATG4D). Phosphorylation by TBK1 on autophagosomes prevents their delipidation by ATG4 and premature removal from nascent autophagosomes. |
| Function | Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruits all ATG8 family members to infecting bacteria such as S.typhimurium. May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins. |
| Protein Name | Microtubule-Associated Protein 1 Light Chain 3 GammaAutophagy-Related Protein Lc3 CAutophagy-Related Ubiquitin-Like Modifier Lc3 CMap1 Light Chain 3-Like Protein 3Microtubule-Associated Proteins 1a/1b Light Chain 3cMap1a/Map1b Lc3 CMap1a/Map1b Light Chain 3 C |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosome MembraneLipid-AnchorEndomembrane SystemCytoplasmCytoskeletonLc3-Ii Binds To The Autophagic Membranes |
| Alternative Antibody Names | Anti-Microtubule-Associated Protein 1 Light Chain 3 Gamma antibodyAnti-Autophagy-Related Protein Lc3 C antibodyAnti-Autophagy-Related Ubiquitin-Like Modifier Lc3 C antibodyAnti-Map1 Light Chain 3-Like Protein 3 antibodyAnti-Microtubule-Associated Proteins 1a/1b Light Chain 3c antibodyAnti-Map1a/Map1b Lc3 C antibodyAnti-Map1a/Map1b Light Chain 3 C antibodyAnti-MAP1LC3C antibody |
Information sourced from Uniprot.org