Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa and C2C12 cells, using Phospho-Stathmin 1-S16 Rabbit pAb (STJ22423) at 1:2000 dilution. HeLa and C2C12 cells were treated by Nocodazole (50ng/mL) for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-STMN1-S16 antibody (STJ22423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 Rabbit pAb (STJ22421) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-STMN1-S25 antibody (STJ22421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of C6, using Phospho-Stathmin 1-S25 Rabbit monoclonal antibody (STJ11105891) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-STMN1-S25 antibody [S5891RM] (STJ11105891)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Stathmin 1-S38 Rabbit pAb (STJ22422) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-STMN1-S38 antibody (STJ22422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-Op18 (S16) Polyclonal Antibody

Anti-Phospho-STMN1-Ser16 antibody (5-54 aa) (STJ90357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Op18 (S25) Polyclonal Antibody

Anti-Phospho-STMN1-Ser25 antibody (9-58 aa) (STJ90358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Op18 (S38) Polyclonal Antibody

Anti-Phospho-STMN1-Ser38 antibody (5-54 aa) (STJ90359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using STMN1 (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-STMN1-Ser62 antibody (26-75 aa) (STJ90936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-STMN2-Ser16 antibody (STJ194748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-STMN2-Ser38 antibody (STJ194749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-STMN2-Ser73 antibody (STJ91270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STX1A-Ser14 antibody (1-50 aa) (STJ90729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~65 kDa Munc18-1 protein phosphorylated at Ser241 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-STXBP1-Ser241 antibody (STJA0003693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Sheep

£454.50

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Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-STXBP1-Ser313 antibody (279-328 aa) (STJ90471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific immunolabeling of the ~65 kDa Munc-18 protein phosphorylated at Ser513 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-STXBP2-Ser 513 antibody (STJA0003694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Bovine/Canine/Chicken/Human/Mouse/Non-Human Primates/Xenopus

£454.50

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-STYK1-Tyr327 antibody (STJA0010008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-SUPT5H/SPT5-S666 antibody (STJ11107712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SYK-Ser297 antibody (STJ196443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells, using SYK (Phospho-Tyr323) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SYK-Tyr323 antibody (289-338 aa) (STJ91056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Syk (Y348) Polyclonal Antibody

Anti-Phospho-SYK-Tyr348 antibody (314-363 aa) (STJ90774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-SYK-Tyr348 antibody (STJ99593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-SYK-Tyr352 antibody (STJ90660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Syk (Y525) Polyclonal Antibody

Anti-Phospho-SYK-Tyr525 antibody (491-540 aa) (STJ90775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-Syk-Tyr525/526 antibody [PT0753R] (STJA0039170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from U-937 cells using Phospho-Syk-Y323 Rabbit pAb (STJ11103715) at1:500 dilution. U-937 cells were treated by ATP (5 mM) at 30C for 1 hour. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.

Anti-Phospho-Syk-Y323 antibody (STJ11103715)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells using Phospho-Syk-Y323 Rabbit pAb (STJ22425). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYK-Y323 antibody (STJ22425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y525/526 Rabbit pAb (STJ113492) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Syk-Y525/526 antibody (STJ113492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (STJ113483) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYK/ZAP70-Y319 antibody (STJ113483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from mouse Brain tissue using Phospho-SYN1-S9 Rabbit pAb (STJ22426). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYN1-S9 antibody (STJ22426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser549 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-SYN1-Ser549 antibody (STJA0003785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Mouse/Rat/Bovine/Canine/Human/Non-Human Primates

£454.50

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Western blot analysis of 293T using SYN1 p-S553 antibody. Antibody was diluted at 1:500

Anti-Phospho-SYN1-Ser553 antibody (STJ97692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-SYN1-Ser603 antibody (STJA0003786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Xenopus/Zebrafish

£454.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using Synapsin1 (Phospho-Ser605) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SYN1-Ser605 antibody (576-625 aa) (STJ91309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells treated with Anisomycin 25ug/ml 30', using Synapsin1 (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SYN1-Ser62 antibody (26-75 aa) (STJ90832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific labeling of the ~78 kDa synapsin protein phosphorylated at Ser62, 67 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-SYN1-Ser62/67 antibody (STJA0003784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine

£454.50

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Western blot analysis of various cells using Phospho-Synapsin I (S9) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SYN1-Ser9 antibody (3-52 aa) (STJ90417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9 (STJA0003783, 1:500, red). Cells and photo courtesy of QBMCellScience.

Anti-Phospho-SYN1-Ser9 antibody (STJA0003783)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Mouse/Rat/Zebrafish/Bovine/Human/Xenopus

£454.50

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Western blot analysis of various cells using Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody

Anti-Phospho-Synaptotagmin 1/2-Ser309/306 antibody (276-325 aa) (STJ90746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Synaptotagmin 1/2 (T202/199) Polyclonal Antibody

Anti-Phospho-Synaptotagmin 1/2-Thr202/199 antibody (176-225 aa) (STJ90747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Synuclein-Alpha-Ser129 antibody [PT1002R] (STJA0040308)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.

Anti-Phospho-SYT1-Ser309 antibody (STJA0003792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Zebrafish

£454.50

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Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (STJA0003791, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at-20C for 10 minutes. Photo courtesy of Gang Liu.

Anti-Phospho-SYT1-Thr202 antibody (STJA0003791)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Zebrafish

£454.50

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Anti-Phospho-TAB2-Ser372 antibody (STJ196444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-TACC3-Ser558 antibody (STJ196445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using (STJ11103659) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-TAK1-S439 antibody [S9MR] (STJ11103659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Anti-Phospho-TAK1-T184 antibody (STJ11107722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using TAL-1 (Phospho-Ser122) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TAL1-Ser122 antibody (96-145 aa) (STJ90638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific immunolabeling of the ~120 kDa TAO2 phosphorylated at Ser181 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-TAOK2-Ser181 antibody (STJA0003794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Canine/Chicken/Finch/Xenopus/Zebrafish

£454.50

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