• Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5', using Shc (Phospho-Tyr427) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using Shc (Phospho-Tyr427) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Shc (Y427) Polyclonal Antibody diluted at 1:500

Anti-Phospho-SHC1-Tyr427 antibody (393-442 aa) (STJ90739)

SKU:
STJ90739

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-SHC-transforming protein 1-Tyr427 (393-442 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHC1
Gene ID: 6464
Uniprot ID: SHC1_HUMAN
Immunogen Region: 393-442 aa
Specificity: Phospho-Shc (Y427) Polyclonal Antibody detects endogenous levels of Shc protein only when phosphorylated at Y427.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Shc around the phosphorylation site of Tyr427 at the amino acid range 393-442
Post Translational Modifications Phosphorylated by activated epidermal growth factor receptor. Phosphorylated in response to FLT4 and KIT signaling. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light. Tyrosine phosphorylated in response to FLT3 signaling. Tyrosine phosphorylated by activated PTK2B/PYK2. Tyrosine phosphorylated by ligand-activated ALK. Tyrosine phosphorylated by ligand-activated PDGFRB. Tyrosine phosphorylated by TEK/TIE2. May be tyrosine phosphorylated by activated PTK2/FAK1.tyrosine phosphorylation was seen in an astrocytoma biopsy, where PTK2/FAK1 kinase activity is high, but not in normal brain tissue. Isoform p52Shc dephosphorylation by PTPN2 may regulate interaction with GRB2.
Function Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span. Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
Protein Name Shc-Transforming Protein 1
Shc-Transforming Protein 3
Shc-Transforming Protein A
Src Homology 2 Domain-Containing-Transforming Protein C1
Sh2 Domain Protein C1
Database Links Reactome: R-HSA-1236382
Reactome: R-HSA-1250196
Reactome: R-HSA-1250347
Reactome: R-HSA-167044
Reactome: R-HSA-180336
Reactome: R-HSA-201556
Reactome: R-HSA-210993
Reactome: R-HSA-2424491 P29353-2
Reactome: R-HSA-2428933
Reactome: R-HSA-2730905 P29353-2
Reactome: R-HSA-2871796 P29353-2
Reactome: R-HSA-2871809 P29353-2
Reactome: R-HSA-354192
Reactome: R-HSA-381038
Reactome: R-HSA-512988
Reactome: R-HSA-5637810
Reactome: R-HSA-5654688
Reactome: R-HSA-5654699
Reactome: R-HSA-5654704
Reactome: R-HSA-5654719
Reactome: R-HSA-5673001
Reactome: R-HSA-74749
Reactome: R-HSA-74751
Reactome: R-HSA-8851805 P29353-2
Reactome: R-HSA-8853659
Reactome: R-HSA-8983432
Reactome: R-HSA-9009391
Reactome: R-HSA-9020558
Reactome: R-HSA-9026519
Reactome: R-HSA-9027284
Reactome: R-HSA-9034864
Reactome: R-HSA-912526
Reactome: R-HSA-9634285
Reactome: R-HSA-9634597
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9674555
Reactome: R-HSA-9680350
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Cell Junction
Focal Adhesion
Isoform P46shc: Mitochondrion Matrix
Localized To The Mitochondria Matrix
Targeting Of Isoform P46shc To Mitochondria Is Mediated By Its First 32 Amino Acids
Which Behave As A Bona Fide Mitochondrial Targeting Sequence
Isoform P52shc And Isoform P66shc
That Contain The Same Sequence But More Internally Located
Display A Different Subcellular Localization
Isoform P66shc: Mitochondrion
In Case Of Oxidative Conditions
Phosphorylation At 'Ser-36' Of Isoform P66shc
Leads To Mitochondrial Accumulation
Alternative Antibody Names Anti-Shc-Transforming Protein 1 antibody
Anti-Shc-Transforming Protein 3 antibody
Anti-Shc-Transforming Protein A antibody
Anti-Src Homology 2 Domain-Containing-Transforming Protein C1 antibody
Anti-Sh2 Domain Protein C1 antibody
Anti-SHC1 antibody
Anti-SHC antibody
Anti-SHCA antibody

Information sourced from Uniprot.org

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