• Western blot analysis of lysates from HeLa cells, using Nonvoltage-gated Sodium Channel 1 (Phospho-Thr615) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of COS7 cells, using Nonvoltage-gated Sodium Channel 1 (Phospho-Thr615) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using Nonvoltage-gated Sodium Channel 1 (Phospho-Thr615) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-SCNN1B-Thr615 antibody (581-630 aa) (STJ91101)

SKU:
STJ91101

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Amiloride-sensitive sodium channel subunit beta channel subunit beta-Thr615 (581-630 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCNN1B
Gene ID: 6338
Uniprot ID: SCNNB_HUMAN
Immunogen Region: 581-630 aa
Specificity: Phospho-ENaC Beta (T615) Polyclonal Antibody detects endogenous levels of ENaC Beta protein only when phosphorylated at T615.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Nonvoltage-gated Sodium Channel 1 around the phosphorylation site of Thr615 at the amino acid range 581-630
Post Translational Modifications Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. N-glycosylated. N-glycosylation is required for interaction with BPIFA1.
Function Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Protein Name Amiloride-Sensitive Sodium Channel Subunit Beta
Beta-Nach
Epithelial Na(+ Channel Subunit Beta
Beta-Enac
Enacb
Nonvoltage-Gated Sodium Channel 1 Subunit Beta
Scneb
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-9730628
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle Membrane
Apical Membrane Of Epithelial Cells
Alternative Antibody Names Anti-Amiloride-Sensitive Sodium Channel Subunit Beta antibody
Anti-Beta-Nach antibody
Anti-Epithelial Na(+ Channel Subunit Beta antibody
Anti-Beta-Enac antibody
Anti-Enacb antibody
Anti-Nonvoltage-Gated Sodium Channel 1 Subunit Beta antibody
Anti-Scneb antibody
Anti-SCNN1B antibody

Information sourced from Uniprot.org

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