Phospho Antibodies | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human brain, using Claudin 5 (Phospho-Tyr217) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CLDN5-Tyr217 antibody (169-218 aa) (STJ91249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using Claudin 6 (Phospho-Tyr219) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CLDN6-Tyr219 antibody (171-220 aa) (STJ90625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from rat liver, using Claudin 7 (Phospho-Tyr210) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CLDN7-Tyr210 antibody (162-211 aa) (STJ90626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CLSPN-Ser30 antibody (STJA0009982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using CNOT2 (Phospho-Ser101) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CNOT2-Ser101 antibody (67-116 aa) (STJ91265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-Cofilin-Ser3 antibody [PT0749R] (STJA0040280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.

Anti-Phospho-Connexin 35/36-Ser276 antibody (STJA0003859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Fish/Mouse/Rabbit

$613.58

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43 kDa connexin43 phosphorylated at Ser368 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 min).

Anti-Phospho-Connexin 43-Ser368 antibody (STJA0003603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Guinea Pig/Human/Zebrafish

$613.58

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Western blot analysis of lysates from K562 cells treated with UV 15', using RFWD2 (Phospho-Ser387) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-COP1-Ser387 antibody (353-402 aa) (STJ90637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-Phospho-CORO1B-Ser-2 antibody (STJA0005349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Western blot analysis of lysates from Jurkat cells using Phospho-Cortactin-Y466 Rabbit pAb (STJ11107623). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Cortactin-Y466 antibody (STJ11107623)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17 kDa COXIV phosphorylated at Ser58 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-COXIV-Ser58 antibody (STJA0003604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

$613.58

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Anti-Phospho-CREB-1-Ser133 antibody [PT0863R] (STJA0039162)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-Phospho-CREB-2-Ser245 antibody (212-261) (STJA0039125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF-P/ELISA

Reactivity :

Human/Rat/Mouse

$0.00

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Anti-Phospho-CREB-2-Ser245 antibody (STJA0039126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$0.00

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Anti-Phospho-CREB1-S119 antibody [P5G10] (STJA0019174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat/Bovine

$510.98 - $2,203.88

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Western blot analysis of lysates from 293T cells, using Phospho-CREB1-S129 Rabbit pAb (STJ11101038) at 1:500 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-CREB1-S129 antibody (STJ11101038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of C6, using Phospho-CREB1-S129 Rabbit polyclonal antibody (STJ11105889) at 1:400 dilution. C6 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-CREB1-S129 antibody (STJ11105889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Western blot analysis of lysates from HeLa cells, using Phospho-CREB1-S133 Rabbit pAb (STJ227724). HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-CREB1-S133 antibody (STJ22076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CREB1-S133 Rabbit pAb (STJ22079) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-CREB1-S133 antibody (STJ22079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemistry analysis of paraffin-embedded rat colon using Phospho-CREB1-S133 Rabbit monoclonal antibody (STJ11105877) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-CREB1-S133 antibody [S5877RM] (STJ11105877)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-Phospho-CREB1-S133 antibody [SAA0304] (STJA0019173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$510.98 - $2,203.88

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Anti-Phospho-CREB1-S142 antibody (STJ11105778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-CREB1-Ser-133 antibody [M415] (STJA0005351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$431.33

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Anti-Phospho-CREB1-Ser-271 antibody (STJA0005352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$493.43

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Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using CREB (Phospho-Ser121) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CREB1-Ser121 antibody (91-140 aa) (STJ90463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CREB (Phospho-Ser129) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CREB1-Ser129 antibody (96-145 aa) (STJ90234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of Hela cell. 1, CREB-1 (phospho Ser133) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-CREB1-Ser133 antibody (100-149 aa) (STJ90235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Pig

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CREB (Phospho-Ser133) antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-Phospho-CREB1-Ser133 antibody (26-140 aa) [R07-3C4] (STJA0011169)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

$332.78

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Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (STJA0003605, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).

Anti-Phospho-CREB1-Ser133 antibody (STJA0003605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Sheep/Xenopus/Zebrafish

$613.58

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Western blot analysis of various cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CREB1-Ser142 antibody (111-160 aa) (STJ91016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HUVEC cells treated with etoposide 25uM 24H, using CREB (Phospho-Thr100) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CREB1-Thr100 antibody (71-120 aa) (STJ90565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-CRK-Tyr-221 antibody (STJA0005354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Anti-Phospho-CRK-Tyr-251 antibody (STJA0005355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Western blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Anti-Phospho-CRK-Tyr221 antibody (187-236 aa) (STJ90687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of lysates from K-562 cells, using Phospho-CRK/CRKII-Y221 Rabbit pAb (STJ117920) at 1:1000 dilution. K-562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody (STJ117920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb (STJ11102643) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody [S3MR] (STJ11102643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-Phospho-CRKL-Tyr-207 antibody (STJA0005357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Western blot analysis of various cells using Phospho-Crk-L (Y207) Polyclonal Antibody

Anti-Phospho-CRKL-Tyr207 antibody (173-222 aa) (STJ91029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-CRKL-Y207 Rabbit pAb (STJ11107726) at1:500 dilution. NIH/3T3 cells were treated by Î»-PP mixed solution (1ul) at 30C for 30 minutes. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 180s.

Anti-Phospho-CRKL-Y207 antibody (STJ11107726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$174.83 - $867.38

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Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit pAb (STJ117922) at 1:1000 dilution or CRKL antibody (STJ230245). K-562 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CRKL-Y207 antibody (STJ117922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-CRMP1-Tyr504 antibody (STJA0009970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-CRMP2-T514/S518 antibody (STJ13100074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$447.53

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Anti-Phospho-CRMP2-T514/S518 antibody (STJ13100794)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$447.53

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CRTC1-Ser151 antibody (STJ196456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-CRTC2-S171 antibody (STJ11107711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-CRY1-S506 antibody (STJ11107694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

$174.83 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using CRYAB (Phospho-Ser19) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CRYAB-Ser19 antibody (10-59 aa) (STJ90895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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