• Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-Che-1/AATF-Ser477 antibody (STJA0003595)

SKU:
STJA0003595-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Bovine/Canine/Mouse/Non-Human Primates/Rat/Sheep/Xenopus
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Che-1/AATF-Ser477 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AATF
Gene ID: 26574
Uniprot ID: AATF_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH.
Tissue Specificity Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Post Translational Modifications Hyperphosphorylated during the G1/S phase transition.
Function Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Protein Name Protein Aatf
Apoptosis-Antagonizing Transcription Factor
Rb-Binding Protein Che-1
Database Links Reactome: R-HSA-193648
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Protein Aatf antibody
Anti-Apoptosis-Antagonizing Transcription Factor antibody
Anti-Rb-Binding Protein Che-1 antibody
Anti-AATF antibody
Anti-CHE1 antibody
Anti-DED antibody
Anti-HSPC277 antibody

Information sourced from Uniprot.org

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