Western Blot Antibodies | St John's Laboratory

Immunofluorescence analysis of U2OS cells using ATG12 Rabbit polyclonal antibody (STJ118058) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG12 antibody (1-100) (STJ118058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ATG12 antibody (20-70 aa) (STJ13100544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

D.melanogaster

£331.50

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Western blot analysis of extracts of various cell lines, using ATG12 antibody (STJ11101729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ATG12 antibody (41-140) [S9MR] (STJ11101729)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-Atg12 antibody at 1:500 dilution; 293HEK cells transfected with GFP-Atg12; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ATG12 antibody (65aa N-Term) (STJ140014)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ATG12 antibody (80-130 aa) (STJ195672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATG12 antibody (STJ13100043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£331.50

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Western blot analysis of 1) Hela Cell, 2) Rat Brain Tissue, 3) Mouse Brainr Tissue lysate using Rabbit pAb diluted at 1:2,000.

Anti-ATG13 antibody (182-232) (STJA0006722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATG13 antibody (281-480 aa) [SRM] (STJ11107161)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using ATG13 antibody (STJ22706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 25s.

Anti-ATG13 antibody (281-517) (STJ22706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using ATG14 Rabbit polyclonal antibody (STJ29664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG14 antibody (340-440) (STJ29664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ATG16L1 antibody (170-210 aa) (STJ13100026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Immunofluorescence analysis of U2OS cells using ATG16L1 Rabbit polyclonal antibody (STJ113881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ATG16L1 antibody (200-300) (STJ113881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry of rat tissue with Anti-ATG16L1 primary antibody (STJ500180) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.

Anti-ATG16L1 antibody (560-610) (STJ500180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-ATG16L1 antibody (560-610) {Biotin} (STJ500181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-ATG16L1 antibody (560-610) {FITC} (STJ500182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit monoclonal antibody (STJ11101913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATG16L1 antibody (6-105) [S3MR] (STJ11101913)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of Jurkat using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG16L1 antibody (60-140 aa) (STJ91756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A-549 cells using ATG16L1 antibody (STJ22707). Blue: DAPI for nuclear staining.

Anti-ATG16L1 antibody (90-300) (STJ22707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ71227 (0. 1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATG16L1 antibody (Internal) (STJ71227)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Anti-ATG16L1 antibody (N-Term) (STJ13100598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Anti-ATG16L1 antibody (STJ13100004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-ATG16L1 antibody (STJ13100556)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Anti-ATG16L2 antibody (1-250 aa) (STJ11106901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using ATG16L2 Rabbit polyclonal antibody (STJ117147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATG16L2 antibody (1-275) (STJ117147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of ATG16L2 Antibody. The lane on the right is blocked with the ATG16L2 peptide.

Anti-ATG16L2 antibody (241-290 aa) (STJ91757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ATG2A Rabbit pAb (STJ111311) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ATG2A antibody (700-800) (STJ111311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ATG2A antibody (STJA0007166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ATG2B Rabbit pAb (STJ110796) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-ATG2B antibody (1360-1600) (STJ110796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ATG2B antibody (266-316) (STJA0006691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using ATG3 antibody (STJ11101718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-ATG3 antibody (1-100) [S8MR] (STJ11101718)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of NIH-3T3 cells using ATG3 Rabbit polyclonal antibody (STJ28372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG3 antibody (1-314) (STJ28372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of ATG3 (green) in Hela using ATG3 antibody, and DAPI (blue)

Anti-ATG3 antibody (2-20 aa) [R06-9E7] (STJA0010998)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£246.50

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Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ATG3 antibody (28-78 aa) (STJ195122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATG3 antibody (STJ13100530)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG3 antibody (STJ13100534)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG3 antibody (STJ13100541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Western blot analysis of ATG3 at dilution of 1:1000

Anti-ATG3 antibody [SM5121] (STJA0005121)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunofluorescence analysis of NIH/3T3 cells using ATG4A Rabbit polyclonal antibody (STJ22708) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG4A antibody (210-398) (STJ22708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KO Validated] ATG4A Rabbit pAb (STJ11106792) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.

Anti-ATG4A antibody (289-398 aa) (STJ11106792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and ATG4A knockout (KO) HeLa cells, using [KO Validated] ATG4A Rabbit polyclonal antibody (STJ11100720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ATG4A antibody (289-398) (STJ11100720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of Colo using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG4A antibody (81-130 aa) (STJ91758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ATG4A antibody (STJ13100548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

£331.50

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Anti-ATG4A antibody (STJ13100553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Anti-ATG4A antibody (STJ13100554)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Western blot analysis of mouse-liver lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-ATG4A antibody (STJ99646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from rat brain, using ATG4B Rabbit pAb (STJ116047) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-ATG4B antibody (1-393) (STJ116047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ATG4B antibody (STJ22639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-ATG4B antibody (1-393) (STJ22639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ATG4B antibody (20-80 aa) (STJ13100547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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