Anti-ARHGEF4 antibody (N-Term) (STJ70430)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ARHGEF4 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 1 |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF4 |
| Gene ID | 50649 |
| Uniprot ID | ARHG4_HUMAN |
| Accession Number | NP_056135.2; NP_127462.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | PWEEPAGEKPSCS |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1). |
Additional Info
| Function | Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 4Apc-Stimulated Guanine Nucleotide Exchange Factor 1AsefAsef1 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Isoform 3: CytoplasmCell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SideAssociated With Membrane Ruffles |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 4 antibodyAnti-Apc-Stimulated Guanine Nucleotide Exchange Factor 1 antibodyAnti-Asef antibodyAnti-Asef1 antibodyAnti-ARHGEF4 antibodyAnti-KIAA1112 antibody |
Information sourced from Uniprot.org