Anti-ARIH2/TRIAD1 antibody (C-Term) (STJ70309)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ARIH2/TRIAD1 (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Applications | Pep-ELISA/WB/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1) Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.Cus |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ARIH2 |
| Gene ID | 10425 |
| Uniprot ID | ARI2_HUMAN |
| Accession Number | NP_006312.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | EQRRRTLLKDFHDT |
Additional Info
| Post Translational Modifications | Ubiquitinated. Ubiquitination promotes proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex. Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB. Promotes ubiquitination of DCUN1D1. Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination. May play a role in myelopoiesis. (Microbial infection) Following infection by HIV-1 virus, acts together with a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, to catalyze ubiquitination and degradation of APOBEC3F and APOBEC3G. |
| Protein Name | E3 Ubiquitin-Protein Ligase Arih2Ari-2Protein Ariadne-2 HomologTriad1 Protein |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Arih2 antibodyAnti-Ari-2 antibodyAnti-Protein Ariadne-2 Homolog antibodyAnti-Triad1 Protein antibodyAnti-ARIH2 antibodyAnti-ARI2 antibodyAnti-TRIAD1 antibodyAnti-HT005 antibody |
Information sourced from Uniprot.org