• Immunofluorescence analysis of L929 cells using ARL1 Rabbit polyclonal antibody (STJ117449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ARL1 Rabbit polyclonal antibody (STJ117449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ARL1 Rabbit polyclonal antibody (STJ117449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ARL1 antibody (62-181) (STJ117449)

SKU:
STJ117449

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARL1 (62-181) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARL1
Gene ID: 400
Uniprot ID: ARL1_HUMAN
Immunogen Region: 62-181
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 62-181 of human ARL1 (NP_001168.1).
Immunogen Sequence: KFQVWDLGGQTSIRPYWRCY YSNTDAVIYVVDSCDRDRIG ISKSELVAMLEEEELRKAIL VVFANKQDMEQAMTSSEMAN SLGLPALKDRKWQIFKTSAT KGTGLDEAMEWLVETLKSRQ
Tissue Specificity Detected in heart, liver, lung and liver (at protein level). Detected in fetal heart, lung, liver and kidney. Detected in adult heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Function GTP-binding protein that recruits several effectors, such as golgins, arfaptins and Arf-GEFs to the trans-Golgi network, and modulates their functions at the Golgi complex. Plays thereby a role in a wide range of fundamental cellular processes, including cell polarity, innate immunity, or protein secretion mediated by arfaptins, which were shown to play a role in maintaining insulin secretion from pancreatic beta cells.
Protein Name Adp-Ribosylation Factor-Like Protein 1
Database Links Reactome: R-HSA-6811440
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Golgi Apparatus
Trans-Golgi Network Membrane
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Adp-Ribosylation Factor-Like Protein 1 antibody
Anti-ARL1 antibody

Information sourced from Uniprot.org

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