Western Blot Antibodies | St John's Laboratory

Whole cell lysates of Jurkat were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD7 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 25 kDaObserved band size: 37 kDa

Anti-CD7 antibody (1-100 aa) [ABT035] (STJ197125)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

€217.50 - €315.00

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STJ72678 (0. 5µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD7 antibody (132-146) (STJ72678)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Western blot analysis of lysates from Jurkat cells, using CD7 Rabbit mAb (STJ11102491) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CD7 antibody (141-240) [S1MR] (STJ11102491)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of various lysates, using CD7 (STJ11105109) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD7 antibody (26-180 aa) [S5109RM] (STJ11105109)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of extracts of Rat spleen, using CD7 antibody (STJ29964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD7 antibody (26-180) (STJ29964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Western blot analysis of K562 cells using CD7 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD7 antibody (51-100 aa) (STJ96607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Flow cytometry:1×10^6 Raji cells were stained with CD70 Rabbit monoclonal antibody (STJ11104003, 1 ug/ml Blue line). Non-fluorescently stained Raji cells were used as blank control (red line).

Anti-CD70 antibody (39-193) [S4003RM] (STJ11104003)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of various lysates using CD70 Rabbit polyclonal antibody (STJ11102782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD70 antibody (47-126) (STJ11102782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of Raji cells using CD70 Rabbit polyclonal antibody (STJ23009) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD70 antibody (47-126) (STJ23009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using CD70 Polyclonal Antibody diluted at 1：2000

Anti-CD70 antibody (68-117 aa) (STJ92139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CD71 antibody [159-3C9] (STJ16101893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

€359.38 - €1,655.63

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Western blot of UV treated HeLa cell lysate showing specific immunolabeling of the ~98 kDa CD71 protein.

Anti-CD71 antibody [DF1513] (STJA0003869)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

€568.13

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Anti-CD71 antibody [EBS-CD-040] (STJ16101124)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

€359.38 - €1,655.63

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Western blot analysis of lysates from HeLa cells, using CD71/Transferrin Receptor Rabbit mAb (STJ11106847) at1:80000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-CD71/Transferrin Receptor antibody (100-250 aa) [SRM] (STJ11106847)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of lysates from Daudi cells, using CD72 Rabbit pAb (STJ111971) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CD72 antibody (120-359) (STJ111971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of rat spleen, mouse brain, mouse spleen lysis using CD72 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-CD72 antibody (170-220 aa) (STJ97662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CD72 antibody (C-Term) (STJA0005712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€569.38

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Anti-CD72 antibody [Bu40] (STJ16101925)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IP/IHC-P/WB

Reactivity :

Human

€926.88

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Anti-CD73 antibody [M064] (STJA0005337)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

€456.88

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Western blot analysis of various lysates, using CD73/NT5E Rabbit mAb (STJ11106951) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Negative control (NC) : MOLT-4<br/>Exposure time: 45s.

Anti-CD73/NT5E antibody (1-100 aa) [SRM] (STJ11106951)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of various lysates using CD74 Rabbit mAb (STJ11102439) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CD74 antibody (1-100) [S9MR] (STJ11102439)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€161.88 - €718.13

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Human appendix tissue was stained with Anti-CD74 (ABT153) Antibody

Anti-CD74 antibody (150-250 aa) [PT1667] (STJ196948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

€217.50 - €315.00

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STJ73085 (0. 01µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD74 antibody (159-171) (STJ73085)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

€390.63

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Western blot analysis of PC12 cells using CD74 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD74 antibody (161-210 aa) (STJ96829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of Mouse thymus, using CD74 Rabbit polyclonal antibody (STJ11105354) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD74 antibody (56-215) (STJ11105354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Anti-CD74 antibody (73-232 aa) [SRM] (STJ11107254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of various lysates using CD74 Rabbit pAb (STJ115893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CD74 antibody (73-232) (STJ115893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of Daudi cells, using CD74 antibody (STJ27634) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-CD74 antibody (73-232) (STJ27634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Anti-CD74 antibody [EBS-CD-041] (STJ16101894)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

€359.38 - €1,655.63

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Anti-CD74 antibody [EBS-O-041] (STJ16101044)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IF/WB

Reactivity :

Human

€468.13

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Western blot analysis of CD74 at dilution of 1:1000

Anti-CD74 antibody [SM4946] (STJA0004946)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€213.13 - €291.88

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Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa CD75 protein.

Anti-CD75 antibody [LN1] (STJA0003870)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

€568.13

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Anti-CD79 antibody [AT107-2] (STJ16100558)

Clonality :

Monoclonal

Host :

Rat

Applications :

WB/IP/FC/ELISA

Reactivity :

Human/Pig/Rat/Tasmanian Devil/Dog/Cat/Human/Koala

€926.88

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Immunohistochemistry analysis of paraffin-embedded human tonsil using CD79a Rabbit monoclonal antibody (STJ11101562) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD79A antibody (1-100) [S2MR] (STJ11101562)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€183.13 - €803.13

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Rat spleen tissue was stained with Anti-CD79a (ABT-CD79a) Antibody

Anti-CD79A antibody (100-226 aa) [ABT-CD79a] (STJ196920)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

€217.50 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CD79A antibody (141-190 aa) (STJ92142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysate from NIH/3T3 cells, using CD79a antibody.

Anti-CD79A antibody (154-203 aa) (STJ92143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of mouse spleen using CD79a Rabbit polyclonal antibody (STJ29796) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD79A antibody (165-225) (STJ29796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CD79a antibody (26-226 aa) [SRM] (STJ11106877)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Anti-CD79a antibody [HM57] (STJ16101926)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IHC-P/WB

Reactivity :

Human/Mouse/Rabbit/Rat/Pig/Bovine/Guinea Pig/Monkey

€926.88

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Western blot analysis of various lysates, using CD79B rabbit monoclonal antibody (STJ11103563) at 1:9000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD79B antibody (1-100) [S3MR] (STJ11103563)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Anti-CD79B antibody (130-229 aa) [SRM] (STJ11106860)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunohistochemistry analysis of paraffin-embedded rat spleen using CD79B Rabbit polyclonal antibody (STJ23012) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD79B antibody (130-229) (STJ23012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of MCF7, L929, K562 cells using CD79b Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD79B antibody (61-110 aa) (STJ96620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from Raji cells, using CD79B Rabbit monoclonal antibody (STJ11105118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD79B antibody [S5118RM] (STJ11105118)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Anti-CD8 alpha antibody (1-50) (STJ505971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€534.38

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Anti-CD8 alpha antibody (1-50) {Biotin} (STJ505972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€660.63

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Anti-CD8 alpha antibody (1-50) {FITC} (STJ505973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€660.63

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