| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Caspase recruitment domain-containing protein 8 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CARD8 |
| Gene ID: |
22900 |
| Uniprot ID: |
CARD8_HUMAN |
| Specificity: |
This antibody detects endogenous levels of CARD8 at Human |
| Immunogen: |
Synthesized peptide derived from human CARD8 |
| Post Translational Modifications | Caspase recruitment domain-containing protein 8: Undergoes autocatalytic processing within the FIIND domain to generate the N-terminal and C-terminal parts, which are associated non-covalently in absence of pathogens and other damage-associated signals. Caspase recruitment domain-containing protein 8, N-terminus: Ubiquitinated by the N-end rule pathway in response to pathogens and other damage-associated signals, leading to its degradation by the proteasome and subsequent release of the cleaved C-terminal part of the protein (Caspase recruitment domain-containing protein 8, C-terminus), which polymerizes and forms the CARD8 inflammasome. (Microbial infection) Proteolytic cleavage by HIV-1 protease in the disordered region and within the ZU5 region of the FIIND domain promotes ubiquitination of the N-terminal part by the N-end rule pathway and degradation by the proteasome, releasing the cleaved C-terminal part of the protein (Caspase recruitment domain-containing protein 8, C-terminus), which polymerizes and forms the CARD8 inflammasome. Isoform 1: Undergoes less autocatalytic processing within the FIIND domain compared to isoform 5. |
| Function | Inflammasome sensor, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis of CD4(+) T-cells and macrophages. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, such as HIV-1 protease activity or Val-boroPro inhibitor, and mediates CARD8 inflammasome activation. In response to pathogen-associated signals, the N-terminal part of CARD8 is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (Caspase recruitment domain-containing protein 8, C-terminus), which polymerizes to initiate the formation of the inflammasome complex: the CARD8 inflammasome directly recruits pro-caspase-1 (proCASP1) independently of PYCARD/ASC and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis. Ability to sense HIV-1 protease activity leads to the clearance of latent HIV-1 in patient CD4(+) T-cells after viral reactivation.in contrast, HIV-1 can evade CARD8-sensing when its protease remains inactive in infected cells prior to viral budding. Also acts as a negative regulator of the NLRP3 inflammasome. May also act as an inhibitor of NF-kappa-B activation. Caspase recruitment domain-containing protein 8: Constitutes the precursor of the CARD8 inflammasome, which mediates autoproteolytic processing within the FIIND domain to generate the N-terminal and C-terminal parts, which are associated non-covalently in absence of pathogens and other damage-associated signals. Caspase recruitment domain-containing protein 8, N-terminus: Regulatory part that prevents formation of the CARD8 inflammasome: in absence of pathogens and other damage-associated signals, interacts with the C-terminal part of CARD8 (Caspase recruitment domain-containing protein 8, C-terminus), preventing activation of the CARD8 inflammasome. In response to pathogen-associated signals, this part is ubiquitinated by the N-end rule pathway and degraded by the proteasome, releasing the cleaved C-terminal part of the protein, which polymerizes and forms the CARD8 inflammasome (Probable). Caspase recruitment domain-containing protein 8, C-terminus: Constitutes the active part of the CARD8 inflammasome. In absence of pathogens and other damage-associated signals, interacts with the N-terminal part of CARD8 (Caspase recruitment domain-containing protein 8, N-terminus), preventing activation of the CARD8 inflammasome. In response to pathogen-associated signals, the N-terminal part of CARD8 is degraded by the proteasome, releasing this form, which polymerizes to form the CARD8 inflammasome complex: the CARD8 inflammasome complex then directly recruits pro-caspase-1 (proCASP1) and promotes caspase-1 (CASP1) activation, leading to gasdermin-D (GSDMD) cleavage and subsequent pyroptosis. |
| Protein Name | Caspase Recruitment Domain-Containing Protein 8Card-Inhibitor Of Nf-Kappa-B-Activating LigandCardinalTumor Up-Regulated Card-Containing Antagonist Of Casp9Tucan Cleaved Into - Caspase Recruitment Domain-Containing Protein 8 - C-TerminusCard8-Ct - Caspase Recruitment Domain-Containing Protein 8 - N-TerminusCard8-Nt |
| Database Links | Reactome: R-HSA-111458Reactome: R-HSA-9627069 |
| Cellular Localisation | CytoplasmNucleusCaspase Recruitment Domain-Containing Protein 8C-Terminus: Inflammasome |
| Alternative Antibody Names | Anti-Caspase Recruitment Domain-Containing Protein 8 antibodyAnti-Card-Inhibitor Of Nf-Kappa-B-Activating Ligand antibodyAnti-Cardinal antibodyAnti-Tumor Up-Regulated Card-Containing Antagonist Of Casp9 antibodyAnti-Tucan Cleaved Into - Caspase Recruitment Domain-Containing Protein 8 - C-Terminus antibodyAnti-Card8-Ct - Caspase Recruitment Domain-Containing Protein 8 - N-Terminus antibodyAnti-Card8-Nt antibodyAnti-CARD8 antibodyAnti-DACAR antibodyAnti-KIAA0955 antibodyAnti-NDPP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
