• Immunohistochemistry analysis of CAPZA2 in paraffin-embedded Mouse kidney using CAPZA2 Rabbit polyclonal antibody (STJ22888) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CAPZA2 in paraffin-embedded Human colon using CAPZA2 Rabbit polyclonal antibody (STJ22888) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CAPZA2 Rabbit polyclonal antibody (STJ22888) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CAPZA2 antibody (1-286) (STJ22888)

SKU:
STJ22888

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAPZA2 (1-286) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPZA2
Gene ID: 830
Uniprot ID: CAZA2_HUMAN
Immunogen Region: 1-286
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human CAPZA2 (NP_006127.1).
Immunogen Sequence: MADLEEQLSDEEKVRIAAKF IIHAPPGEFNEVFNDVRLLL NNDNLLREGAAHAFAQYNLD QFTPVKIEGYEDQVLITEHG DLGNGKFLDPKNRICFKFDH LRKEATDPRPCEVENAVESW RTSVETALRAYVKEHYPNGV CTVYGKKIDGQQTIIACIES HQFQAKNFWNGRWRSEWKFT ITPSTTQVVGILKIQVHYYE DGNVQLVSHKDIQDSLTVSN EVQTAKEFIKIVEAAENEY
Function F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Protein Name F-Actin-Capping Protein Subunit Alpha-2
Capz Alpha-2
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-3371497
Reactome: R-HSA-6807878
Reactome: R-HSA-6811436
Reactome: R-HSA-879415
Reactome: R-HSA-9662360
Reactome: R-HSA-983231
Alternative Antibody Names Anti-F-Actin-Capping Protein Subunit Alpha-2 antibody
Anti-Capz Alpha-2 antibody
Anti-CAPZA2 antibody

Information sourced from Uniprot.org

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