• STJ70336 staining (1µg/ml) of Human Jurkat cells (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
  • STJ70336 (2µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-CARD11 antibody (C-Term) (STJ70336)

SKU:
STJ70336-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CARD11 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IHC-2-5µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CARD11
Gene ID: 84433
Uniprot ID: CAR11_HUMAN
Immunogen Region: C-Term
Accession Number: NP_115791.3
Immunogen Sequence: QRKTIWVDEDQL
Post Translational Modifications Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
Function Adapter protein that plays a key role in adaptive immune response by transducing the activation of NF-kappa-B downstream of T-cell receptor (TCR) and B-cell receptor (BCR) engagement. Transduces signals downstream TCR or BCR activation via the formation of a multiprotein complex together with BCL10 and MALT1 that induces NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways. Upon activation in response to TCR or BCR triggering, CARD11 homooligomerizes to form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10 and subsequent recruitment of MALT1: this leads to I-kappa-B kinase (IKK) phosphorylation and degradation, and release of NF-kappa-B proteins for nuclear translocation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Promotes linear ubiquitination of BCL10 by promoting the targeting of BCL10 to RNF31/HOIP. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway.
Protein Name Caspase Recruitment Domain-Containing Protein 11
Card-Containing Maguk Protein 1
Carma 1
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-202424
Reactome: R-HSA-2871837
Reactome: R-HSA-5607764
Cellular Localisation Cytoplasm
Membrane Raft
Colocalized With Dpp4 In Membrane Rafts
Alternative Antibody Names Anti-Caspase Recruitment Domain-Containing Protein 11 antibody
Anti-Card-Containing Maguk Protein 1 antibody
Anti-Carma 1 antibody
Anti-CARD11 antibody
Anti-CARMA1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance