Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates, using CD59 Rabbit monoclonal antibody (STJ11104908) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD59 antibody (26-126 aa) [S4908RM] (STJ11104908)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using CD59 antibody (STJ114286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD59 antibody (26-128) (STJ114286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of CD59 in paraffin-embedded human placenta using CD59 Rabbit polyclonal antibody (STJ23006) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD59 antibody (26-128) (STJ23006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse testis using CD59 Rabbit monoclonal antibody (STJ11101994) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD59 antibody (49-128) [S4MR] (STJ11101994)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of K562 cells using CD59 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD59 antibody (51-100 aa) (STJ96617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CD59 antibody [M011] (STJA0005325)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

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Anti-CD59 antibody [M015] (STJA0005326)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

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Anti-CD59 antibody [MEM-43/5] (STJ16100089)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/IP/IHC-P

Reactivity :

Mouse/Human

£405.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD5L antibody (220-300 aa) (STJ191677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of the lysates from HT-29 cells using CD6 antibody.

Anti-CD6 antibody (250-299 aa) (STJ92136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using CD6 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD6 antibody (361-410 aa) (STJ97272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CD6 Rabbit pAb (STJ110406) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-CD6 antibody (424-668) (STJ110406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CD6 antibody [MEM-98] (STJ16100729)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-P

Reactivity :

Human

£405.50

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Anti-CD61 antibody [Y2/51] (STJ16101921)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IF/IHC-P/WB

Reactivity :

Human

£741.50

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Anti-CD62E/E-Selectin antibody (22-230 aa) [SRM] (STJ11107318)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CD62L/SELL antibody [DREG56] (STJA0001439)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£93.50 - £114.50

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Anti-CD62P antibody [AK-6] (STJ16101922)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IF/IHC-P/WB

Reactivity :

Human

£741.50

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Western blot analysis of various lysates using CD63 Rabbit monoclonal antibody (STJ11104602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD63 antibody (100-200) [S4602RM] (STJ11104602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of A431 cells using CD63 Rabbit polyclonal antibody (STJ11105705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD63 antibody (103-203) (STJ11105705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of CD63 in paraffin-embedded human tonsil using CD63 Rabbit monoclonal antibody (STJ11101561) at dilution of 1:10000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD63 antibody (103-203) [S1MR] (STJ11101561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-CD63 antibody at 1:2500 dilution lysate at 50 µg per lane Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CD63 antibody (120aa to 175aa) (STJ140029)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Immunofluorescence analysis of human-breast-cancer tissue. 1, CD63 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-CD63 antibody (121-170 aa) (STJ96890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CD63 antibody (Extracellular region) [M034] (STJA0005327)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

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Anti-CD63 antibody (Extracellular region) [M040] (STJA0005328)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£365.50

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Western blot analysis of various lysates using CD63 Rabbit pAb (STJ11106733) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-CD63 antibody (STJ11106733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of CD63 at dilution of 1:1000

Anti-CD63 antibody [SM0504] (STJA0010504)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£170.50 - £233.50

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Anti-CD66a/CEACAM1 antibody [SRM] (STJ11107209)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of HepG2, mouse brain cells using CD66c/d Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-CD66c/d antibody (31-80 aa) (STJ97289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of RAW264. 7 cells using CD68 Rabbit polyclonal antibody (STJ28637) at dilution of 1:250. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD68 antibody (100-200) (STJ28637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and CD68 Rabbit polyclonal antibody knockout (KO) U-87MG cells, using [KO Validated] CD68 Rabbit polyclonal antibody (STJ11104713) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD68 antibody (16-200) (STJ11104713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunofluorescence analysis of THP-1 cells using CD68 Rabbit polyclonal antibody (STJ117231) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD68 antibody (16-200) (STJ117231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of mouse spleen cells using CD68 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD68 antibody (171-220 aa) (STJ96575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-CD68 antibody (22-319) (STJ115250)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot with Anti-CD68 Antibody (STJ500480) of mouse liver at 1:500 dilution in buffer.

Anti-CD68 antibody (240-290) (STJ500480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CD68 antibody (240-290) {Biotin} (STJ500481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CD68 antibody (240-290) {FITC} (STJ500482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Human liver tissue was stained with Anti-CD68 (ABT-CD68) Antibody

Anti-CD68 antibody (250-354 aa) [ABT-CD68] (STJ196750)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of extracts of RAW264. 7 cells, using CD68 antibody (STJ11103378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD68 antibody (50-150) (STJ11103378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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STJ73443 (1µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD68 antibody (Internal) (STJ73443)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human

£312.50

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Anti-CD68 antibody [KP1] (STJ16101923)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IP/IHC-P/WB

Reactivity :

Human

£741.50

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Anti-CD68 antibody [SRM] (STJ11106991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using CD69 antibody (STJ23008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD69 antibody (1-80) (STJ23008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using CD69 Polyclonal Antibody diluted at 1：1000

Anti-CD69 antibody (101-150 aa) (STJ92138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CD69 antibody (62-199 aa) [SRM] (STJ11107231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of rat spleen using CD69 Rabbit monoclonal antibody (STJ11104741) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD69 antibody (62-199) [S4741RM] (STJ11104741)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of HepG2 cells using CD69 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD69 antibody (91-140 aa) (STJ96828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using CD69 monoclonal antibody against, Jurkat (1) , L1210 (2) and TPH-1 (3) cell lysate.

Anti-CD69 antibody [8B6] (STJ97933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Whole cell lysates of Jurkat were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD7 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 25 kDaObserved band size: 37 kDa

Anti-CD7 antibody (1-100 aa) [ABT-CD7] (STJ196816)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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