• Immunohistochemistry analysis of paraffin-embedded rat kidney using Calpain 2 Rabbit polyclonal antibody (STJ11105674) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Calpain 2 Rabbit polyclonal antibody (STJ11105674) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Calpain 2 Rabbit polyclonal antibody (STJ11105674) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Calpain 2 antibody (STJ11105674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of various cell lines, using Calpain 2 antibody (STJ11105674). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CAPN2 antibody (520-700) (STJ11105674)

SKU:
STJ11105674

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Calpain-2 Catalytic Subunit (520-700) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPN2
Gene ID: 824
Uniprot ID: CAN2_HUMAN
Immunogen Region: 520-700
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 520-700 of human Calpain 2 (NP_001739.2).
Immunogen Sequence: LEEFDISEDDIDDGFRRLFA QLAGEDAEISAFELQTILRR VLAKRQDIKSDGFSIETCKI MVDMLDSDGSGKLGLKEFYI LWTKIQKYQKIYREIDVDRS GTMNSYEMRKALEEAGFKMP CQLHQVIVARFADDQLIIDF DNFVRCLVRLETLFKIFKQL DPENTGTIELDLISWLCFSV L
Tissue Specificity Ubiquitous.
Function Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
Protein Name Calpain-2 Catalytic Subunit
Calcium-Activated Neutral Proteinase 2
Canp 2
Calpain M-Type
Calpain Large Polypeptide L2
Calpain-2 Large Subunit
Millimolar-Calpain
M-Calpain
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Cell Membrane
Translocates To The Plasma Membrane Upon Ca(2+) Binding
Alternative Antibody Names Anti-Calpain-2 Catalytic Subunit antibody
Anti-Calcium-Activated Neutral Proteinase 2 antibody
Anti-Canp 2 antibody
Anti-Calpain M-Type antibody
Anti-Calpain Large Polypeptide L2 antibody
Anti-Calpain-2 Large Subunit antibody
Anti-Millimolar-Calpain antibody
Anti-M-Calpain antibody
Anti-CAPN2 antibody
Anti-CANPL2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance