• Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Calpain small subunit 1 antibody (STJ28622) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Calpain small subunit 1 antibody (STJ28622) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using Calpain small subunit 1 antibody (STJ28622) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Calpain small subunit 1 Antibody (STJ28622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CAPNS1 antibody (69-268) (STJ28622)

SKU:
STJ28622

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAPNS1 (69-268) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPNS1
Gene ID: 826
Uniprot ID: CPNS1_HUMAN
Immunogen Region: 69-268
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 69-268 of human Calpain small subunit 1 (NP_001740.1).
Immunogen Sequence: ISEAAAQYNPEPPPPRTHYS NIEANESEEVRQFRRLFAQL AGDDMEVSATELMNILNKVV TRHPDLKTDGFGIDTCRSMV AVMDSDTTGKLGFEEFKYLW NNIKRWQAIYKQFDTDRSGT ICSSELPGAFEAAGFHLNEH LYNMIIRRYSDESGNMDFDN FISCLVRLDAMFRAFKSLDK DGTGQIQVNIQEWLQLTMYS
Function Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development.
Protein Name Calpain Small Subunit 1
Css1
Calcium-Activated Neutral Proteinase Small Subunit
Canp Small Subunit
Calcium-Dependent Protease Small Subunit
Cdps
Calcium-Dependent Protease Small Subunit 1
Calpain Regulatory Subunit
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-6809371
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Cell Membrane
Translocates To The Plasma Membrane Upon Calcium Binding
Alternative Antibody Names Anti-Calpain Small Subunit 1 antibody
Anti-Css1 antibody
Anti-Calcium-Activated Neutral Proteinase Small Subunit antibody
Anti-Canp Small Subunit antibody
Anti-Calcium-Dependent Protease Small Subunit antibody
Anti-Cdps antibody
Anti-Calcium-Dependent Protease Small Subunit 1 antibody
Anti-Calpain Regulatory Subunit antibody
Anti-CAPNS1 antibody
Anti-CAPN4 antibody
Anti-CAPNS antibody

Information sourced from Uniprot.org

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