• Western blot analysis of extracts of various cell lines, using Calpain 1 rabbit monoclonal antibody (STJ11102361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 Rabbit monoclonal antibody (STJ11102361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Calpain 1 rabbit monoclonal antibody (STJ11102361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Calpain 1 Rabbit monoclonal antibody (STJ11102361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Calpain 1 rabbit monoclonal antibody (STJ11102361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Calpain 1 Rabbit monoclonal antibody (STJ11102361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CAPN1 antibody (100-200) [S1MR] (STJ11102361)

SKU:
STJ11102361

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Calpain 1 (100-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPN1
Gene ID: 823
Uniprot ID: CAN1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Calpain 1 (P07384).
Immunogen Sequence: DGATRTDICQGALGDCWLLA AIASLTLNDTLLHRVVPHGQ SFQNGYAGIFHFQLWQFGEW VDVVVDDLLPIKDGKLVFVH SAEGNEFWSALLEKAYAKVN G
Tissue Specificity Ubiquitous.
Post Translational Modifications Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
Function Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).
Protein Name Calpain-1 Catalytic Subunit
Calcium-Activated Neutral Proteinase 1
Canp 1
Calpain Mu-Type
Calpain-1 Large Subunit
Cell Proliferation-Inducing Gene 30 Protein
Micromolar-Calpain
Mucanp
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-6798695
Reactome: R-HSA-6809371
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Cell Membrane
Translocates To The Plasma Membrane Upon Ca(2+) Binding
In Granular Keratinocytes And In Lower Corneocytes
Colocalizes With Flg And Flg2
Alternative Antibody Names Anti-Calpain-1 Catalytic Subunit antibody
Anti-Calcium-Activated Neutral Proteinase 1 antibody
Anti-Canp 1 antibody
Anti-Calpain Mu-Type antibody
Anti-Calpain-1 Large Subunit antibody
Anti-Cell Proliferation-Inducing Gene 30 Protein antibody
Anti-Micromolar-Calpain antibody
Anti-Mucanp antibody
Anti-CAPN1 antibody
Anti-CANPL1 antibody
Anti-PIG30 antibody

Information sourced from Uniprot.org

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