Immunofluorescence Antibodies | St John's Laboratory

Anti-EIF2AK4 antibody (1600-1700) {FITC} (STJ501141)

Clonality :

Polyclonal

Host :

Chicken

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-EIF2AK4 antibody (865-914 aa) (STJ93239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using EIF2B1 Rabbit pAb (STJ110201) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-EIF2B1 antibody (1-305) (STJ110201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2B2 antibody (1-120) (STJ29107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using EIF2B3 antibody (STJ112300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B3 antibody (1-270) (STJ112300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using EIF2B4 Rabbit polyclonal antibody (STJ11100161) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2B4 antibody (30-100) (STJ11100161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) (EIF2B5) Rabbit mAb (STJ11102350) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-EIF2B5 antibody (1-100) [S0MR] (STJ11102350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301). Western blot was performed from the immunoprecipitate using eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301) at a dilution of 1:1000.

Anti-EIF2B5 antibody (442-721) (STJ112301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-EIF2S1 antibody (21-70 aa) (STJ92872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Fish

£48.00 - £252.00

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Western blot detection of eIF2 Alpha in K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela cell lysates using eIF2 Alpha mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Anti-EIF2S1 antibody [2E4-F5-E7] (STJ99266)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of EIF2A at dilution of 1:1000

Anti-EIF2S1 antibody [SM5103] (STJA0005103)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EIF2S2 antibody (10-90 aa) (STJ92873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using EIF3A antibody (STJ29809) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-EIF3A antibody (1-250) (STJ29809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using eIF3B Polyclonal Antibody (STJ112297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF3B antibody (100-350) (STJ112297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using eIF3B Rabbit mAb (STJ11102435) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EIF3B antibody (101-200) [S5MR] (STJ11102435)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of U2OS cells using EIF3C antibody (STJ29102). Blue: DAPI for nuclear staining.

Anti-EIF3C antibody (694-913) (STJ29102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-EIF3D antibody (1-240) (STJ27743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EIF3D Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF3D antibody (101-150 aa) (STJ92879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using eIF3 Epsilon Polyclonal Antibody

Anti-EIF3F antibody (81-130 aa) (STJ92878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa, using EIF3F rabbit polyclonal antibody (STJ29103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-EIF3F antibody (88-357) (STJ29103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (STJ23512) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF3G antibody (1-320) (STJ23512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 1 Mu g EIF3H antibody (STJ115340). Western blot was performed from the immunoprecipitate using EIF3H antibody (STJ115340) at a dilution of 1:1000.

Anti-EIF3H antibody (80-340) (STJ115340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and EIF3H knockout (KO) HeLa cells, using [KO Validated] EIF3H Rabbit polyclonal antibody (STJ119999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF3H antibody (80-340) (STJ119999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using EIF3J Rabbit pAb (STJ113577) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-EIF3J antibody (1-258) (STJ113577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using eIF3L Polyclonal Antibody diluted at 1：1000

Anti-EIF3L antibody (1-50 aa) (STJ92876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-EIF4A1 antibody (1-100) (STJ27247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-EIF4A1 antibody [M8] (STJ97038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using eIF4A Rabbit polyclonal antibody (STJ11103240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF4A1/EIF4A2/EIF4A3 antibody (1-100) (STJ11103240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using EIF4B Rabbit pAb (STJ11107417) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EIF4B antibody (352-611 aa) (STJ11107417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of Rat skeletal muscle, using EIF4B antibody (STJ115264) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-EIF4B antibody (352-611) (STJ115264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells, using eIF4B Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4B antibody (388-437 aa) (STJ92880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using eIF4E antibody (STJ23516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-EIF4E antibody (1-100) (STJ23516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of MCF7 cells using eIF4E antibody (STJ114889). Blue: DAPI for nuclear staining.

Anti-EIF4E antibody (1-217) (STJ114889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using eIF4E Polyclonal Antibody diluted at 1：1000

Anti-EIF4E antibody (168-217 aa) (STJ92881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis using eIF4E monoclonal antibody against HeLa (1) , HEK293 (2) and K562 (3) cell lysate.

Anti-EIF4E antibody [5D11] (STJ98016)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of U-2 OS cells using EIF4E2 Rabbit polyclonal antibody (STJ26609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF4E2 antibody (1-240) (STJ26609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using 4E-BP1 Polyclonal Antibody diluted at 1：500

Anti-EIF4EBP1 antibody (13-62 aa) (STJ91383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Sheep

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4EBP1 antibody (2-51 aa) (STJ91387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4EBP1 antibody (30-79 aa) (STJ91384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4EBP1 antibody (4-53 aa) (STJ91386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using eIF4EBP1 antibody (STJ29842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF4EBP1 antibody (50-118) (STJ29842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein (1).

Anti-EIF4EBP1 antibody [11G12C11] (STJ97805)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2).

Anti-EIF4EBP1 antibody [4B6G10] (STJ97804)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of U2OS cells using EIF4ENIF1 Rabbit polyclonal antibody (STJ117369) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF4ENIF1 antibody (150-250) (STJ117369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells, using eIF4G Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4G1 antibody (1074-1123 aa) (STJ92883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g EIF4G2/p97 antibody (STJ23521). Western blot was performed from the immunoprecipitate using EIF4G2/p97 antibody (STJ23521) at a dilution of 1:1000.

Anti-EIF4G2 antibody (320-490) (STJ23521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using DAP-5 Polyclonal Antibody

Anti-EIF4G2 antibody (41-90 aa) (STJ92651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using EIF5 antibody (STJ28666). Blue: DAPI for nuclear staining.

Anti-EIF5 antibody (1-235) (STJ28666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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