• Immunofluorescence analysis of paraffin-embedded Mouse heart using CHRNA1 Rabbit polyclonal antibody (STJ27259) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of CHRNA1 in paraffin-embedded Human skeletal muscle tissue using CHRNA1 Rabbit polyclonal antibody (STJ27259) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using CHRNA1 Rabbit polyclonal antibody (STJ27259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHRNA1 antibody (105-220) (STJ27259)

SKU:
STJ27259

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRNA1 (105-220) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRNA1
Gene ID: 1134
Uniprot ID: ACHA_HUMAN
Immunogen Region: 105-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 105-220 of human CHRNA1 (NP_000070.1).
Immunogen Sequence: IWRPDLVLYNNADGDFAIVK FTKVLLQYTGHITWTPPAIF KSYCEIIVTHFPFDEQNCSM KLGTWTYDGSVVAINPESDQ PDLSNFMESGEWVIKESRGW KHSVTYSCCPDTPYLD
Tissue Specificity Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.
Function Isoform 1: Upon acetylcholine binding, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Isoform 2: Non functional acetylcholine receptor alpha subunit which is not integrated into functional acetylcholine-gated cation-selective channels.
Protein Name Acetylcholine Receptor Subunit Alpha
Database Links Reactome: R-HSA-629594
Reactome: R-HSA-629597
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Acetylcholine Receptor Subunit Alpha antibody
Anti-CHRNA1 antibody
Anti-ACHRA antibody
Anti-CHNRA antibody

Information sourced from Uniprot.org

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