Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, IL-1 Beta Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL1B antibody (181-230 aa) (STJ96566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of recombinant mouse IL-1 beta protein, using IL1 Beta Rabbit monoclonal antibody (STJ11104780) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-IL1Beta antibody (118-269) [S4780RM] (STJ11104780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western blot analysis of MCF7 cells using IL-1RI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R1 antibody (31-80 aa) (STJ96628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of PC12 cells using IL-1RII Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R2 antibody (141-190 aa) (STJ96835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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STJ72269 (0. 5µg/ml) staining of LNCaP (A) and (1ug/ml) MOLT-4 (B) cell-lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IL1R2 antibody (Internal) (STJ72269)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Confocal imaging of mouse tail ear using IL1RA Rabbit monoclonal antibody (STJ11101277, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-IL1RN antibody (100-177) [S7MR] (STJ11101277)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of A-431 cells, using IL1RA antibody (STJ24179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL1RN antibody (24-177) (STJ24179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IL2 antibody [SRM13481] (STJ11108211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Anti-IL2 antibody [SRM15021] (STJ11109751)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using (STJ29315) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL21 antibody (25-162) (STJ29315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IL21 antibody [SRM13293] (STJ11108023)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Rat

£126.50 - £554.50

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Anti-IL33 antibody (109-270 aa) [SRM5370] (STJ11107280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of HeLa cells using IL-33 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL33 antibody (121-170 aa) (STJ96852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-IL33 antibody [SRM14549] (STJ11109279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200

Anti-IL37 antibody (101-150 aa) (STJ98774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of HepG2 cells using IL-3R Alpha Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL3RA antibody (241-290 aa) (STJ97265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using IL-4R Alpha Polyclonal Antibody diluted at 1：2000

Anti-IL4R antibody (463-512 aa) (STJ93702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of AD293, 4T1 cells using IL-6R Alpha Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL6R antibody (221-270 aa) (STJ97241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using CD127/IL7R rabbit monoclonal antibody (STJ11101386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL7R antibody (300-400) [S6MR] (STJ11101386)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from THP-1 cells using IL8 Rabbit mAb (STJ11107026) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight and LPS (1 Mu g/ml) at 37C for 6 hours. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-IL8 antibody (28-99 aa) [SRM5168] (STJ11107026)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £554.50

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Anti-IL8 antibody [SRM14770] (STJ11109500)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g ILF3 antibody (STJ24197). Western blot was performed from the immunoprecipitate using ILF3 antibody (STJ24197) at a dilution of 1:1000.

Anti-ILF3 antibody (1-270) (STJ24197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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ILF3 antibody western blot HeLa lysates STJA0036909

Anti-ILF3 antibody [R02-5S3] (STJA0036909)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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ILF3/NF90 antibody western blot Jurkat C6 HeLa STJA0034469

Anti-ILF3 antibody [R04-3C1] (STJA0034469)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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ILF3 antibody immunocytochemistry Jurkat cells STJA0034468

Anti-ILF3 antibody [R04-9E8] (STJA0034468)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-IMP-3 antibody [PT1304R] (STJA0040099)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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STJ72253 (0. 3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IMP1/ZBP1 antibody (Internal) (STJ72253)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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IMP3 antibody western blot K562 rat brain C6 3T3 HeLa STJA0034470

Anti-IMP3 antibody [R04-7F2] (STJA0034470)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of A549 cells using IMPA1 antibody (STJ28464). Blue: DAPI for nuclear staining.

Anti-IMPA1 antibody (1-277) (STJ28464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-IMPA1/IMP1 antibody (1-277aa) [AT2G5] (STJA0041776)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-Importin Alpha 5/KPNA1 antibody [SRM14717] (STJ11109447)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Indoleamine 2, 3-dioxygenase antibody [R03-5D4] (STJA0034471)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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INDO antibody western blot HeLa interferon gamma STJA0036493

Anti-Indoleamine 2, 3-dioxygenase antibody [R08-4C3] (STJA0036493)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Immunofluorescence analysis of L929 cells using INMT Rabbit polyclonal antibody (STJ116603) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-INMT antibody (1-263) (STJ116603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of mouse spleen using SHIP11 Rabbit monoclonal antibody (STJ11101903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-INPP5D antibody (1084-1178) [S3MR] (STJ11101903)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of THP-1 cells using SHIP1 Rabbit polyclonal antibody (STJ117994) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-INPP5D antibody (900-1000) (STJ117994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of L929 cells using INPP5E Rabbit polyclonal antibody (STJ119776) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-INPP5E antibody (510-630) (STJ119776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using INPP5J antibody (STJ28714). Blue: DAPI for nuclear staining.

Anti-INPP5J antibody (60-360) (STJ28714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-INPP5J antibody (850-930 aa) (STJ93716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Immunofluorescence analysis of PC-12 cells using INPP5K Rabbit polyclonal antibody (STJ110117) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-INPP5K antibody (149-448) (STJ110117)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100

Anti-INPPL1 antibody (351-400 aa) (STJ96591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-INPPL1 antibody [8C13] (STJ16103500)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£746.50

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Confocal imaging of human pancreas using Insulin Rabbit monoclonal antibody (STJ11101603, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-INS antibody (1-100) [S3MR] (STJ11101603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using Insulin antibody (STJ24210) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-INS antibody (1-110) (STJ24210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-Insulin (ABT-INS) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Insulin recombinant proteinPredicted band size: 12kDaObserved band size: 9kDa

Anti-INS antibody (25-110 aa) [ABT-INS] (STJ196791)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-Insulin (ABT340) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Insulin recombinant proteinPredicted band size: 12kDaObserved band size: 9kDa

Anti-INS antibody (25-110 aa) [PT0156] (STJ196713)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-Insulin (ABT189) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Insulin recombinant proteinPredicted band size: 12kDaObserved band size: 9kDa

Anti-INS antibody (25-110 aa) [PT2015] (STJ196973)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human-pancreas, antibody was diluted at 1:100

Anti-INS antibody (30-70 aa) (STJ98980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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