• Western blot analysis of extracts of various cell lines, using (STJ29315) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat spleen, using (STJ29315) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using IL21 rabbit polyclonal antibody (STJ29315) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using IL21 rabbit polyclonal antibody (STJ29315) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using IL21 rabbit polyclonal antibody (STJ29315) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using IL21 rabbit polyclonal antibody (STJ29315) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using IL21 rabbit polyclonal antibody (STJ29315) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL21 antibody (25-162) (STJ29315)

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STJ29315

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL21 (25-162) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL21
Gene ID: 59067
Uniprot ID: IL21_HUMAN
Immunogen Region: 25-162
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-162 of human IL21 (NP_068575.1).
Immunogen Sequence: HKSSSQGQDRHMIRMRQLID IVDQLKNYVNDLVPEFLPAP EDVETNCEWSAFSCFQKAQL KSANTGNNERIINVSIKKLK RKPPSTNAGRRQKHRLTCPS CDSYEKKPPKEFLERFKSLL QKMIHQHLSSRTHGSEDS
Tissue Specificity Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
Function Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
Protein Name Interleukin-21
Il-21
Za11
Database Links Reactome: R-HSA-9020958
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-21 antibody
Anti-Il-21 antibody
Anti-Za11 antibody
Anti-IL21 antibody

Information sourced from Uniprot.org

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