• Western blot analysis of extracts of various cell lines, using CD127/IL7R rabbit monoclonal antibody (STJ11101386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Confocal imaging of human spleen using CD127/IL7R rabbit monoclonal antibody (STJ11101386, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Confocal imaging of mouse spleen using CD127/IL7R rabbit monoclonal antibody (STJ11101386, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-IL7R antibody (300-400) [S6MR] (STJ11101386)

SKU:
STJ11101386

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IL7R alpha/CD127 (300-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL7R
Gene ID: 3575
Uniprot ID: IL7RA_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CD127/IL7R (P16871).
Immunogen Sequence: PESFLDCQIHRVDDIQARDE VEGFLQDTFPQQLEESEKQR LGGDVQSPNCPSEDVVITPE SFGRDSSLTCLAGNVSACDA PILSSSRSLDCRESGKNGPH V
Post Translational Modifications N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
Function Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Protein Name Interleukin-7 Receptor Subunit Alpha
Il-7 Receptor Subunit Alpha
Il-7r Subunit Alpha
Il-7r-Alpha
Il-7ra
Cdw127
Cd Antigen Cd127
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 3: Cell Membrane
Isoform 4: Secreted
Alternative Antibody Names Anti-Interleukin-7 Receptor Subunit Alpha antibody
Anti-Il-7 Receptor Subunit Alpha antibody
Anti-Il-7r Subunit Alpha antibody
Anti-Il-7r-Alpha antibody
Anti-Il-7ra antibody
Anti-Cdw127 antibody
Anti-Cd Antigen Cd127 antibody
Anti-IL7R antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance