Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-SFN antibody (41-90 aa) (STJ91377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells using SH2B1 antibody (STJ27510). Blue: DAPI for nuclear staining.

Anti-SH2B1 antibody (360-520) (STJ27510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, SIRT1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SIRT1 antibody (13-62 aa) (STJ95667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (STJ25533) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT1 antibody (229-246) (STJ25533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SIRT1 antibody (448-747) (STJ119437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using SIRT1 monoclonal antibody against MCF-7 (1) , Jurkat (2) , HeLa (3) , HEK293 (4) and A549 (5) cell lysate.

Anti-SIRT1 antibody [1F3] (STJ98380)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Monkey

£149.00 - £360.00

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Anti-SIRT1 antibody [PT0158R] (STJA0039739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Rat

£150.00 - £540.00

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Western blot analysis of Rat brain, using SIRT2 antibody (STJ114449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-SIRT2 antibody (300-350) (STJ114449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of NIH/3T3 cells using SIRT2 Rabbit polyclonal antibody (STJ25534) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT2 antibody (300-350) (STJ25534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot detection of SirT2 in PC-12, Rat Brain and Mouse Brain cell lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39, 43KDa. Observed band size:39, 43KDa.

Anti-SIRT2 antibody [1D4-H11-H11] (STJ99093)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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SirT2 antibody western blot PC-12 rat mouse brain STJA0032378

Anti-SIRT2 antibody [1D4-H11-H11] (STJA0032378)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using SIRT7 Rabbit polyclonal antibody (STJ25536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SIRT7 antibody (201-400) (STJ25536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of K562 cells using SLAM Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SLAMF1 antibody (81-130 aa) (STJ96644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of A549, K562 cells using Smad1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SMAD1 antibody (150-230 aa) (STJ96712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMAD1 antibody (20-240) (STJ11104508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Smad1 antibody [PT0687R] (STJA0039763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Smad1 antibody western blot 293T cells STJA0036149

Anti-Smad1 antibody [R02-1J1] (STJA0036149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Smad1 antibody western blot HeLa lysates STJA0035864

Anti-Smad1 antibody [R03-4B4] (STJA0035864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Smad1 antibody [SRM13316] (STJ11108046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of lysate from K562 cells, using Brg-1 antibody.

Anti-SMARCA4 antibody (1565-1614 aa) (STJ91889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using BRG1/SMARCA4 antibody (STJ11101695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SMARCA4 antibody (200-300) [S5MR] (STJ11101695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HeLa cells using BRG1/BRG1/SMARCA4 antibody (STJ113555).

Anti-SMARCA4 antibody (30-130) (STJ113555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g BRG1/SMARCA4 antibody (STJ25612). Western blot was performed from the immunoprecipitate using BRG1/SMARCA4 antibody (STJ25612) at a dilution of 1:1000.

Anti-SMARCA4 antibody (680-770) (STJ25612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot detection of BRG1 in Hela NE, F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.

Anti-SMARCA4 antibody [6D7-F7-B6] (STJ99234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis using SorLA monoclonal antibody against truncated SorLA recombinant protein.

Anti-SORL1 antibody [3B6B11] (STJ98393)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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STJ70018 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic with endosome staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SORL1/LR11 antibody (C-Term) (STJ70018)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Immunofluorescence analysis of U2OS cells using STAMBP Rabbit polyclonal antibody (STJ29145). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-STAMBP antibody (100-270) (STJ29145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Sulfotransferase 2A1 antibody western blot human fetal liver STJA0037547

Anti-Sulfotransferase 2A1 antibody (STJA0037547)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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SULT2A1 antibody western blot HepG2 STJA0036870

Anti-Sulfotransferase 2A1 antibody [R04-9L1] (STJA0036870)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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ICC IF of SULT2A1 in Hep3B cells stained with STJA0041864 antibody

Anti-Sulfotransferase 2A1/SULT2A1 antibody (1-285aa) [AT13E10] (STJA0041864)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of various lysates using SULT1A3 Rabbit polyclonal antibody (STJ114235) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SULT1A3 antibody (1-100) (STJ114235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U-2 OS cells using SULT1C4 Rabbit polyclonal antibody (STJ117628) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SULT1C4 antibody (1-95) (STJ117628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A549 cells using SULT1E1 antibody (STJ29588). Blue: DAPI for nuclear staining.

Anti-SULT1E1 antibody (140-294) (STJ29588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Hep G2 cells, using SULT2A1 Rabbit mAb (STJ11103622) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-SULT2A1 antibody (180-285) [S2MR] (STJ11103622)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SULT2A1 antibody (STJ193153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using SULT2B1 Rabbit polyclonal antibody (STJ110047) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SULT2B1 antibody (1-365) (STJ110047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit polyclonal antibody (STJ11100173) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TBC1D4 antibody (1059-1298) (STJ11100173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells, using AS160 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TBC1D4 antibody (611-660 aa) (STJ95925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-TBC1D4 antibody [PT0652R] (STJA0039306)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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STJ72725 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TERT antibody (597-611) (STJ72725)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of HeLa, HepG2 cells using TERT Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-TERT antibody (931-980 aa) (STJ96879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-TERT antibody [PT1186R] (STJA0040213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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p53 antibody western blot Jurkat lysates STJA0011734

Anti-TP53 antibody (1-101 aa) [R07-6A7] (STJA0011734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Immunoprecipitation of p53 in 200 µg extracts from 293T cells using 3 µg p53 Rabbit polyclonal antibody (STJ25926). Western blot analysis was performed using p53 Rabbit polyclonal antibody (STJ25926) at 1:3000 dilution.

Anti-TP53 antibody (1-393) (STJ25926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 1) p53 knockout A431 cell, 2) A431 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-TP53 antibody (10-59 aa) (STJ94893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Human esophageal squamous cell carcinoma tissue was stained with Anti-P53 (ABT-P53) Antibody

Anti-TP53 antibody (250-393 aa) [ABT-P53] (STJ197083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-P53 (ABT002) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 53kDaObserved band size: 53kDa

Anti-TP53 antibody (250-393 aa) [ABT002] (STJ197180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti TP53 Goat Polyclonal antibody immunofluorescence STJ140047

Anti-TP53 antibody (280aa C-Term) (STJ140047)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £440.50

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