• Western blot analysis of extracts of various cell lines, using CALB2 antibody (STJ111849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of mouse brain using Calretinin Rabbit polyclonal antibody (STJ111849) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CALB2 antibody (STJ111849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of rat brain using Calretinin Rabbit polyclonal antibody (STJ111849) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat brain using CALB2 rabbit polyclonal antibody (STJ111849) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Calretinin Rabbit polyclonal antibody (STJ111849) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human brain using CALB2 rabbit polyclonal antibody (STJ111849) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain using Calretinin Rabbit polyclonal antibody (STJ111849) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using CALB2 rabbit polyclonal antibody (STJ111849) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Calretinin Rabbit polyclonal antibody (STJ111849) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat brain using CALB2 rabbit polyclonal antibody (STJ111849) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Calretinin antibody (STJ111849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of mouse brain using CALB2 rabbit polyclonal antibody (STJ111849) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Calretinin antibody (STJ111849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CALB2 antibody (1-271) (STJ111849)

SKU:
STJ111849

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CALB2 (1-271) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALB2
Gene ID: 794
Uniprot ID: CALB2_HUMAN
Immunogen Region: 1-271
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human Calretinin (NP_001731.2).
Immunogen Sequence: MAGPQQQPPYLHLAELTASQ FLEIWKHFDADGNGYIEGKE LENFFQELEKARKGSGMMSK SDNFGEKMKEFMQKYDKNSD GKIEMAELAQILPTEENFLL CFRQHVGSSAEFMEAWRKYD TDRSGYIEANELKGFLSDLL KKANRPYDEPKLQEYTQTIL RMFDLNGDGKLGLSEMSRLL PVQENFLLKFQGMKLTSEEF NAIFTFYDKDRSGYIDEHEL DALLKDLYEKNKKEMNIQQ
Tissue Specificity Brain.
Function Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Protein Name Calretinin
Cr
29 Kda Calbindin
Alternative Antibody Names Anti-Calretinin antibody
Anti-Cr antibody
Anti-29 Kda Calbindin antibody
Anti-CALB2 antibody
Anti-CAB29 antibody

Information sourced from Uniprot.org

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