Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using Phospho-Tau-T181 Rabbit pAb (STJ11101032) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-MAPT-T498 antibody (STJ11101032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S166 Rabbit pAb (STJ22283). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MDM2-S166 antibody (STJ22283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Anti-Phospho-MECP2-Ser-421 antibody (STJA0005465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

Choose Options

Anti-Phospho-MECP2-Ser-80 antibody (STJA0005464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

$493.43

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-MEK3-S189 Rabbit pAb (STJ11107632). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MEK3-S189 antibody (STJ11107632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

$174.83 - $867.38

Choose Options

Western blot with Anti-Phospho-MERTK Antibody (STJ502558) on mouse macrophage. Antibody at 1:800 dilution.

Anti-Phospho-MERTK antibody (746-757) (STJ502558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/FC/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

Choose Options

Anti-Phospho-MERTK antibody (746-757) {Biotin} (STJ502559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/FC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$789.08

Choose Options

Anti-Phospho-MERTK antibody (746-757) {FITC} (STJ502560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/FC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$789.08

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-mTOR-S2448 Rabbit pAb (STJ27512) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-mTOR-S2448 antibody (STJ27512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Anti-Phospho-MYH9-S1943 antibody (STJA0005473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

Choose Options

Anti-Phospho-MYL12A-Ser-1 antibody (STJA0005479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Bovine

$496.13

Choose Options

Anti-Phospho-MYL12A-Ser-19 antibody (STJA0005480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

Choose Options

Anti-Phospho-N/A-p-Tyr antibody (STJA0005529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

$431.33

Choose Options

Anti-Phospho-N/A-p-Tyr antibody [M375] (STJA0005528)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

$431.33

Choose Options

Anti-Phospho-N/A-pSer/Thr antibody (STJA0005526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Anti-Phospho-N/A-pSer/Thr antibody [M380A/M380B] (STJA0005525)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Anti-Phospho-N/A-pThr antibody (STJA0005527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$431.33

Choose Options

Immunofluorescence analysis of Hep G2 cells using Phospho-NRF2-S40 Rabbit pAb (STJ11105795) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-NFE2L2-S40 antibody (STJ11105795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-NFKB1-S893 antibody (STJ22310).

Anti-Phospho-NFKB1-S893 antibody (STJ22310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from MDA-MB435 cells, using Phospho-NF-Kappa B2-S866 Rabbit pAb (STJ22313) at dilution. MDA-MB435 cells were treated by TNF-Alpha. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NFKB2-S866 antibody (STJ22313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-I Kappa B Alpha-S32 Rabbit pAb (STJ116373) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-NFKBIA-S32 antibody (STJ116373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Anti-Phospho-NGEF-Tyr-87 antibody (STJA0005409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$493.43

Choose Options

Anti-Phospho-NOS2-Tyr-1055 antibody (STJA0005496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Anti-Phospho-NOS3-Tyr-657 antibody (STJA0005492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Immunohistochemistry of rat tissue with Anti-Phospho-PDE11A primary antibody (S117) (STJ502594) at 1:50 dilution in buffer. Section was treated with DAB and Haematoxylin stain.

Anti-Phospho-PDE11A antibody (75-125) (STJ502594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

Choose Options

Anti-Phospho-PDE11A antibody (75-125) {Biotin} (STJ502597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

Choose Options

Anti-Phospho-PDE11A antibody (75-125) {FITC} (STJ502598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

Choose Options

Western blot with Anti-Phospho-PDE5A Antibody (STJ502621) and recombinant PDE5A1. Overexpressing cells were treated with or without ANP. Antibody at 1:500 dilution in buffer.

Anti-Phospho-PDE5A antibody (120-170) (STJ502621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IHC/IP/WB

Reactivity :

Bovine/Human/Mouse/Pig/Rat/Sheep

$713.48

Choose Options

Anti-Phospho-PDE5A antibody (120-170) {Biotin} (STJ502622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IHC/IP/WB

Reactivity :

Bovine/Human/Mouse/Pig/Rat/Sheep

$789.08

Choose Options

Anti-Phospho-PDE5A antibody (120-170) {FITC} (STJ502623)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IHC/IP/WB

Reactivity :

Bovine/Human/Mouse/Pig/Rat/Sheep

$789.08

Choose Options

Anti-Phospho-PDK1-Tyr-9 antibody (STJA0005521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 Rabbit pAb (STJ22329) at 1:1000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-PDPK1-S241 antibody (STJ22329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 pAb (STJ29323) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PDPK1-S241 antibody (STJ29323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKM2-Y105 Rabbit pAb (STJ245505). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PKM-Y105 antibody (STJ11101058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from NIH/3T3 cells using Phospho-PKR/EIF2AK2-T446 Rabbit pAb (STJ11103768) at 1:2000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PKR/EIF2AK2-T446 antibody (STJ11103768)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

Choose Options

Anti-Phospho-PLCG1-Tyr-775 antibody (STJA0005537)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (STJ11102585). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Phospho-POLR2A-S2 antibody [S5MR] (STJ11102585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (STJ11102586) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody [S6MR] (STJ11102586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Anti-Phospho-PRKCA-S657/Y658 antibody (STJA0005533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Rabbit/Rat

$496.13

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 pAb (STJ22341) at 1:1000 dilution or PRKCA antibody (STJ298482). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PRKCA/PRKCB-T638/641 antibody (STJ22341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-DNA-PKcs-S2056 Rabbit pAb (STJ113503). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKDC-S2056 antibody (STJ113503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit pAb (STJ22357) at 1:1000 dilution. Jurkat cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PTK2B-Y402 antibody (STJ22357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Anti-Phospho-PTPN1-Ser-50 antibody (STJA0005553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-SHP1-Y536 antibody (STJ22360).

Anti-Phospho-PTPN6-Y536 antibody (STJ22360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Anti-Phospho-PXN-Ser-83 antibody (STJA0005515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Mouse

$496.13

Choose Options

Anti-Phospho-Pycard-Tyr-144 antibody (STJA0005271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (STJ110827) at 1:400 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-RELA-S276 antibody (STJ110827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (STJ22377) at 1:10000 dilution. HeLa cells were treated by TNF-Alpha (50 ng/ml) and Calyculin A (50 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RELA-S536 antibody (STJ22377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options