Anti-HTRA1 antibody [R22-3C-3] (STJA0035612)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human htrA1
STJA0035612
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General Information

Short DescriptionRabbit monoclonal anti-HTRA1 for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ICC/IF
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR22-3C-3
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
IF 1:50-1:200
FormulationLiquid in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolHTRA1
Gene ID5654
Uniprot IDHTRA1_HUMAN
ImmunogenA synthesized peptide derived from human htrA1

Additional Info

Tissue Specificity Widely expressed, with strongest expression in placenta (at protein level). Secreted by synovial fibroblasts. Up-regulated in osteoarthritis and rheumatoid arthritis synovial fluids and cartilage as compared with non-arthritic (at protein level).
Function Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
Protein Name Serine Protease Htra1
High-Temperature Requirement A Serine Peptidase 1
L56
Serine Protease 11
Database Links Reactome: R-HSA-1474228
Cellular Localisation Cell Membrane
Secreted
Cytoplasm
Cytosol
Predominantly Secreted
Also Found Associated With The Plasma Membrane
Alternative Antibody Names Anti-Serine Protease Htra1 antibody
Anti-High-Temperature Requirement A Serine Peptidase 1 antibody
Anti-L56 antibody
Anti-Serine Protease 11 antibody
Anti-HTRA1 antibody
Anti-HTRA antibody
Anti-PRSS11 antibody

Information sourced from Uniprot.org

Citations

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