• Immunohistochemistry analysis of paraffin-embedded mouse brain using HTATSF1 antibody (STJ27773) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using HTATSF1 antibody (STJ27773) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using HTATSF1 antibody (STJ27773) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HTATSF1 antibody (STJ27773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HTATSF1 antibody (1-270) (STJ27773)

SKU:
STJ27773

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HTATSF1 (1-270) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTATSF1
Gene ID: 27336
Uniprot ID: HTSF1_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HTATSF1 (NP_055315.2).
Immunogen Sequence: MSGTNLDGNDEFDEQLRMQE LYGDGKDGDTQTDAGGEPDS LGQQPTDTPYEWDLDKKAWF PKITEDFIATYQANYGFSND GASSSTANVEDVHARTAEEP PQEKAPEPTDARKKGEKRKA ESGWFHVEEDRNTNVYVSGL PPDITVDEFIQLMSKFGIIM RDPQTEEFKVKLYKDNQGNL KGDGLCCYLKRESVELALKL LDEDEIRGYKLHVEVAKFQL KGEYDASKKKKKCKDYKKK
Tissue Specificity Widely expressed.
Function Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.
Protein Name Hiv Tat-Specific Factor 1
Tat-Sf1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hiv Tat-Specific Factor 1 antibody
Anti-Tat-Sf1 antibody
Anti-HTATSF1 antibody

Information sourced from Uniprot.org

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