• Immunofluorescence analysis of U2OS cells using HTR2B antibody (STJ27637). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using HTR2B Rabbit polyclonal antibody (STJ27637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HTR2B antibody (382-481) (STJ27637)

SKU:
STJ27637

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HTR2B (382-481) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTR2B
Gene ID: 3357
Uniprot ID: 5HT2B_HUMAN
Immunogen Region: 382-481
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 382-481 of human HTR2B (NP_000858.3).
Immunogen Sequence: LFNKTFRDAFGRYITCNYRA TKSVKTLRKRSSKIYFRNPM AENSKFFKKHGIRNGINPAM YQSPMRLRSSTIQSSSIILL DTLLLTENEGDKTEEQVSYV
Tissue Specificity Ubiquitous. Detected in liver, kidney, heart, pulmonary artery, and intestine. Detected at lower levels in blood, placenta and brain, especially in cerebellum, occipital cortex and frontal cortex.
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine.
Protein Name 5-Hydroxytryptamine Receptor 2b
5-Ht-2b
5-Ht2b
Serotonin Receptor 2b
Database Links Reactome: R-HSA-390666
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Synapse
Synaptosome
Alternative Antibody Names Anti-5-Hydroxytryptamine Receptor 2b antibody
Anti-5-Ht-2b antibody
Anti-5-Ht2b antibody
Anti-Serotonin Receptor 2b antibody
Anti-HTR2B antibody

Information sourced from Uniprot.org

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