• Anti-HUS1 antibody (1-280) (STJ27360)
  • Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g HUS1 antibody (STJ27360). Western blot was performed from the immunoprecipitate using HUS1 antibody (STJ27360) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using HUS1 Rabbit polyclonal antibody (STJ27360). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using HUS1 Rabbit polyclonal antibody (STJ27360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HUS1 antibody (1-280) (STJ27360)

SKU:
STJ27360

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HUS1 (1-280) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HUS1
Gene ID: 3364
Uniprot ID: HUS1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).
Immunogen Sequence: MKFRAKIVDGACLNHFTRIS NMIAKLAKTCTLRISPDKLN FILCDKLANGGVSMWCELEQ ENFFNEFQMEGVSAENNEIY LELTSENLSRALKTAQNARA LKIKLTNKHFPCLTVSVELL SMSSSSRIVTHDIPIKVIPR KLWKDLQEPVVPDPDVSIYL PVLKTMKSVVEKMKNISNHL VIEANLDGELNLKIETELVC VTTHFKDLGNPPLASESTHE DRNVEHMAEVHIDIRKLLQ
Tissue Specificity Ubiquitous.
Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds.endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths.and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.
Protein Name Checkpoint Protein Hus1
Hhus1
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-5685938
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Cytoplasm
Cytosol
In Discrete Nuclear Foci Upon Dna Damage
Localized Also In The Cytoplasm
Dna Damage Induces Its Nuclear Translocation
Shuttles Between The Nucleus And The Cytoplasm
Alternative Antibody Names Anti-Checkpoint Protein Hus1 antibody
Anti-Hhus1 antibody
Anti-HUS1 antibody

Information sourced from Uniprot.org

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