Primary Antibodies | St John's Laboratory

Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95 (red). Photo courtesy of Gang Liu.

Anti-GRIN2B antibody (N-Term) (STJA0003718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates

£454.50

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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit.

Anti-GRIN2A antibody (N-Term) (STJA0003713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Mouse/Rat/Bovine/Canine

£454.50

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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.

Anti-GRIN2A antibody (C-Term) (STJA0003711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP

Reactivity :

Human/Mouse/Rabbit/Rat

£454.50

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Anti-GRIN2A antibody (C-Term) (STJA0003710)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP

Reactivity :

Human/Mouse/Rabbit/Rat

£454.50

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Western blot of rat cortical lysate showing specific immunolabeling of the ~65 kDa Munc-18 protein phosphorylated at Ser513 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-STXBP2-Ser 513 antibody (STJA0003694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Bovine/Canine/Chicken/Human/Mouse/Non-Human Primates/Xenopus

£454.50

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR2/3 protein.

Anti-GRIA2/3 antibody (C-Term) (STJA0003664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Rat/Chicken/Human/Mouse/Zebrafish

£454.50

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-GRIA1-Ser845 antibody (STJA0003662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat/Human/Non-Human Primates

£454.50

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~67 kDa GAT-1 protein.

Anti-GABA Transporter/GAT 1 antibody (C-Term) (STJA0003654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Non-Human Primates/Rat

£454.50

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Western blot of mouse brain lysates from wild type (WT) and Alpha 3-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa Alpha 3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from Alpha 3-knockout animals.

Anti-GABRA3 antibody (N-Term) (STJA0003642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat/Bovine/Canine/Human/Non-Human Primates/Zebrafish

£454.50

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Western blot of mouse brain lysates from wild type (WT) and Alpha 2-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa Alpha 2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from Alpha 2-knockout animals.

Anti-GABRA2 antibody (Cytoplasmic Loop) (STJA0003641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Mouse/Bovine/Canine/Human/Non-Human Primates/Rat/Zebrafish

£454.50

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Immunostaining of a frozen section of paraformaldehyde-fixed mouse brain showing specific immunolabeling of DDC (1:500) in red and fluorescent Nissl (blue). Photo courtesy of Tom Finger, University of Colorado School of Medicine.

Anti-DOPA Decarboxylase antibody (STJA0003612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

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Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-PPP1R1B-Thr34 antibody (STJA0003607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates

£454.50

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Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 (STJA0003606, green, 1:250) and Tuj1 (red). Cells and photo courtesy of QBM CellScience.

Anti-PPP1R1B antibody (N-Term) (STJA0003606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Xenopus

£454.50

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Western blot of rat lung lysate showing specific immunolabeling of the ~180 kDa collagen 1.

Anti-Collagen 1, alpha 1 propeptide antibody (STJA0003599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Feline/Finch/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole/Xenopus

£454.50

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Western blot of rat cerebellar lysate showing specific immunolabeling of the ~29 kDa calretinin protein.

Anti-CALB2 antibody (STJA0003588)

Clonality :

Polyclonal

Host :

Sheep

Applications :

WB/IHC

Reactivity :

Rat/Human/Mouse/Non-Human Primates

£454.50

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Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58 (STJA0003567, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.

Anti-Phospho-14-3-3-Ser58 antibody (STJA0003567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Sheep/Xenopus/Zebrafish

£454.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Beclin 1-Ser93 Rabbit pAb (STJ11103786) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-Beclin 1-S93 antibody (STJ11103786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 Rabbit pAb (STJ11103784) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-Ezrin & Radixin & Moesin-T567 & T564 & T558 antibody (STJ11103784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FGFR3-Y724 Rabbit pAb (STJ11103781) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-FGFR3-S129 antibody (STJ11103781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-AKT1-S129 Rabbit pAb (STJ11103780) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S129 antibody (STJ11103780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit pAb (STJ11103779) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S308 & S309 & S305 antibody (STJ11103779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-IRF7-S471/472 Rabbit pAb (STJ11103778) at 1:500 dilution. 293T and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-IRF7-S471/472 antibody (STJ11103778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-ULK1-S467 Rabbit pAb (STJ11103776) at 1:1000 dilution. HeLa and Jurkat cells were treated by Serum-starvation overnight at 37 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-ULK1-S467 antibody (STJ11103776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Rabbit pAb (STJ11103773) at 1:500 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK14 & MAPK11 & MAPK13 & MAPK12-T180/Y182 & T180/Y182 & T180/Y182 & T183/Y1845 antibody (STJ11103773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells using Phospho-Beclin 1-Ser90 Rabbit pAb (STJ11103771) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Beclin 1-S90 antibody (STJ11103771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-p53-T81 Rabbit pAb (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (STJ11103769) at 1:500 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Beclin 1-S15 antibody (STJ11103769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-PDHA1-S293 Rabbit pAb (STJ11103767) at 1:1000 dilution. 293T cells and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PDHA1-S293 antibody (STJ11103767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-Tau-T181 Rabbit pAb (STJ11103760) at 1:600 dilution. Mouse brain cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Tau-T181 antibody (STJ11103760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-Tau-T217 Rabbit pAb (STJ11103759) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-Tau-T217 antibody (STJ11103759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-CGAS-S420 Rabbit pAb (STJ11103756) at 1:1000 dilution. 293T and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CGAS-S420 antibody (STJ11103756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit pAb (STJ11103753) at 1:1000 dilution. HeLa cells were treated by Nocodazole (100 ng/ml) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MEK1-T292 antibody (STJ11103753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (STJ11103751) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T93 antibody (STJ11103751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-MAP3K5-S967 Rabbit pAb (STJ11103750) at 1:1000 dilution. Jurkat cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-MAP3K5-S967 antibody (STJ11103750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-AKT1-T308 Rabbit pAb (STJ11103747) at 1:1000 dilution. HeLa cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-T308 antibody (STJ11103747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1173 Rabbit pAb (STJ11103746) at 1:500 dilution. A-431 cells were treated by EGF (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-EGFR-Y1193 antibody (STJ11103746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-ULK1-S638 Rabbit pAb (STJ11103735) at 1:1000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ULK1-S638 antibody (STJ11103735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-NCF4/p40-phox-T154 Rabbit pAb (STJ11103733) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-NCF4/p40-T154 antibody (STJ11103733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MCM2-S139 Rabbit pAb (STJ11103732) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. HeLa cells were treated by Serum-starvation overnight at 37 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MCM2-S139 antibody (STJ11103732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-LRP6-S1490 Rabbit pAb (STJ11103731) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-LRP6-S1490 antibody (STJ11103731)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-HSF1-S326 Rabbit pAb (STJ11103729) at 1:500 dilution. MCF7 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-HSF1-S326 antibody (STJ11103729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-LAT-Y191 Rabbit pAb (STJ11103728) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 Mu g/mL) at 37 °C for 2 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-LAT-Y191 antibody (STJ11103728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-YAP1-S128 Rabbit pAb (STJ11103727) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-YAP1-S128 antibody (STJ11103727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-PKC delta-S645 pAb (STJ11103726) at 1:1000 dilution or PKC delta antibody (STJ29804). 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PKC delta-S645 antibody (STJ11103726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PLA2G4A-S505 Rabbit pAb (STJ11103723) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PLA2G4A-S505 antibody (STJ11103723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-NMDAR1-S896/S897 Rabbit pAb (STJ11103722) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-NMDAR1-S896/S897 antibody (STJ11103722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-PLN-S16/T17 Rabbit pAb (STJ11103720) at 1:1000 dilution. Hela cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PLN-S16/T17 antibody (STJ11103720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-TrkA-Y674/675 + TrkB-Y706/707 Rabbit pAb (STJ11103716) at 1:500 dilution. HeLa cells were treated by Pervanadate (1 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-TrkA & TrkB-Y674/675 & Y706/707 antibody (STJ11103716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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