• STJ70957 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70957 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-PTCH antibody (Internal) (STJ70957)

SKU:
STJ70957-100

$409.73
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Host: Goat
Applications: Pep-ELISA/IF/WB
Reactivity: Human/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PTCH (Internal) is suitable for use in ELISA, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IF-Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. 5µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PTCH1
Gene ID: 5727
Uniprot ID: PTC1_HUMAN
Immunogen Region: Internal
Accession Number: NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1
Specificity: This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).
Immunogen Sequence: HPESRHHPPSNPRQQ
Post Translational Modifications Glycosylation is necessary for SHH binding. In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.
Function Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
Protein Name Protein Patched Homolog 1
Ptc
Ptc1
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-5610787
Reactome: R-HSA-5632681
Reactome: R-HSA-5632684
Reactome: R-HSA-5635838
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Protein Patched Homolog 1 antibody
Anti-Ptc antibody
Anti-Ptc1 antibody
Anti-PTCH1 antibody
Anti-PTCH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance