• STJ71105 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71105 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-TMP21/TMED10 antibody (C-Term) (STJ71105)

SKU:
STJ71105-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TMP21/TMED10 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:1000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TMED10
Gene ID: 10972
Uniprot ID: TMEDA_HUMAN
Immunogen Region: C-Term
Accession Number: NP_006818.3
Immunogen Sequence: YLRRFFKAKKLIE
Protein Name Transmembrane Emp24 Domain-Containing Protein 10
Protein Tmed10
21 Kda Transmembrane-Trafficking Protein
S31i125
S31iii125
Tmp-21-I
Transmembrane Protein Tmp21
P23
P24 Family Protein Delta-1
P24delta1
P24delta
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Golgi Apparatus Membrane
Golgi Apparatus
Cis-Golgi Network Membrane
Trans-Golgi Network Membrane
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Cell Membrane
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Transmembrane Emp24 Domain-Containing Protein 10 antibody
Anti-Protein Tmed10 antibody
Anti-21 Kda Transmembrane-Trafficking Protein antibody
Anti-S31i125 antibody
Anti-S31iii125 antibody
Anti-Tmp-21-I antibody
Anti-Transmembrane Protein Tmp21 antibody
Anti-P23 antibody
Anti-P24 Family Protein Delta-1 antibody
Anti-P24delta1 antibody
Anti-P24delta antibody
Anti-TMED10 antibody
Anti-TMP21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance