St John's Laboratory Ltd

Western blot analysis of various lysates using WSTF Rabbit mAb (STJ11102480) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-BAZ1B antibody (1-100) [S0MR] (STJ11102480)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Western blot analysis of lysates from Mouse kidney, using WRAP53 Rabbit polyclonal antibody (STJ11103198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WRAP53 antibody (62-160) (STJ11103198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€132.60 - €606.60

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WSTF antibody western blot SH-SY5Y STJA0036878

Anti-WSTF antibody [R06-4I5] (STJA0036878)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Immunohistochemistry analysis of paraffin-embedded human colon using BRD4 Rabbit monoclonal antibody (STJ11101416) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-BRD4 antibody (100-200) [S6MR] (STJ11101416)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-APBB1 antibody (400-480 aa) (STJ190544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-BRD4 antibody (STJA0008652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-BRD4-T204 Rabbit pAb (STJ11101157) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-BRD4-T204 antibody (STJ11101157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various lysates using Phospho-BRD4-S1083 Rabbit mAb (STJ11103761) at1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 300s.

Anti-Phospho-BRD4-S1083 antibody [S1MR] (STJ11103761)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€172.20 - €742.20

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-BRD4-T249 Rabbit polyclonal antibody (STJ11105910) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-BRD4-T249 antibody (STJ11105910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Recombinant-APBB1 antibody [RM1C68] (STJA0022864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human

€360.60 - €549.00

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Human BRD4 protein (Recombinant) (N-His-SUMO & C-Strep) (STJP015398)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

€250.20 - €1,396.20

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BRD4 antibody western blot HeLa lysates STJA0035921

Anti-BRD4 antibody [R01-1H8] (STJA0035921)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of extracts of various cell lines, using APBB1 antibody (STJ26186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-APBB1 antibody (359-708) (STJ26186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€132.60 - €606.60

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Anti-BRD4 antibody [SAA2122] (STJA0022323)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IP

Reactivity :

Human

€234.60 - €1,583.40

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Human BRD4 protein (Recombinant) (N-10*His-Flag) (STJP014286)

Host :

E.coli

Applications :

ELISA/WB

€545.40

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Dot-blot analysis of all sorts of peptides using Phospho-BRD4-S492/S494 antibody (STJ11102665) at 1:1000 dilution.

Anti-Phospho-BRD4-S492/S494 antibody (STJ11102665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S498/S499 Rabbit pAb (STJ11102666) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-BRD4-S498/S499 antibody (STJ11102666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of lysates from Mouse brain, using Acetyl-BRD4-K332 Rabbit polyclonal antibody (STJ11103227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Acetyl-BRD4-K332 antibody (STJ11103227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€151.80 - €742.20

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Western blot analysis of various lysates using (STJ11103782) at1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-BRD4-T204 antibody [S2MR] (STJ11103782)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€142.20 - €625.80

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FE65 antibody immunocytochemistry 293T cells STJA0012374

Anti-APBB1 antibody (697-710 aa) [R02-0C4] (STJA0012374)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

€160.20 - €337.80

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Western blot analysis of extracts of HeLa cells, using BRD4 antibody (STJ11101530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BRD4 antibody (700-794) (STJ11101530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Dot-blot analysis of all sorts of peptides using Phospho-BRD4-S484/S488 antibody (STJ11102664) at 1:1000 dilution.

Anti-Phospho-BRD4-S484/S488 antibody (STJ11102664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Anti-BRD4 IP antibody [SAA0192] (STJA0018707)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP/IP-MS

Reactivity :

Human

€234.60 - €1,583.40

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Anti-BRD4 antibody [PT1026R] (STJA0039853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S1070 Rabbit pAb (STJ11102668) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-BRD4-S1070 antibody (STJ11102668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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STJ70979 (0. 1µg/ml) staining of NIH/3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-APBB1/FE65 antibody (C-Term) (STJ70979)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€375.00

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STJ73492 (1µg/ml) staining of Mouse Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-BRD4 antibody (Internal) (STJ73492)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

€375.00

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Anti-Recombinant-APBB1 antibody [RM1C67] (STJA0022863)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human

€360.60 - €549.00

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Anti-Recombinant-BRD4 antibody [RM1B66] (STJA0026836)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€360.60 - €549.00

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STJ70352 (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

Anti-EDD1/HYD antibody (C-Term) (STJ70352)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

€375.00

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Anti-Phospho-ATR-S428-S428 antibody [SRM15157] (STJ11109887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of lysates from 293T cells, using Phospho-ATR-S428 Rabbit pAb (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody (STJ114848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-ATR-Thr1989 antibody (STJ196248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of COLO cells using ATR Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ATR antibody (394-443 aa) (STJ91781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Recombinant-UBR5 antibody [RM1G20] (STJA0023212)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€360.60 - €549.00

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Anti-Phospho-ATR-Ser428 antibody [PT1243R] (STJA0040115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of various lysates, using ATR rabbit polyclonal antibody (STJ115886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATR antibody (400-500) (STJ115886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of various cells using UBR5 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-UBR5 antibody (1-50 aa) (STJ96174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Anti-ATR antibody [SAA1672] (STJA0020100)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€238.20 - €1,404.60

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Human ATR protein (Recombinant) (N-His) (STJP005340)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

€250.20 - €1,396.20

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Anti-ATR antibody [PT0864R] (STJA0040070)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of extracts of NCI-H460 cells, using ATR Antibody (STJ29390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATR antibody (400-650) (STJ29390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€132.60 - €606.60

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATR (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATR-Ser428 antibody (394-443 aa) (STJ91234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from HeLa cells, using Phospho-ATR-T1989 Rabbit pAb (STJ11103765) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATR-T1989 antibody (STJ11103765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of extracts of various cell lines, using ATR antibody (STJ11104244) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ATR antibody (2100-2200) [S4244RM] (STJ11104244)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Western blot analysis of various lysates, using Phospho-ATR-S428 antibody (STJ11105842) at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody [S5842RM] (STJ11105842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-ATR antibody [SRM14487] (STJ11109217)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€142.20 - €625.80

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Human PRKDC protein (Recombinant) (N-His-GST) (STJP014581)

Host :

E.coli

Applications :

ELISA/WB

€400.20

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